Antibacterial Peptide CecropinB2 Production via Various Host and Construct Systems

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Wei-Shiang LaiYung-Chuan Liu

Abstract

Cecropin is a cationic antibacterial peptide composed of 35-39 residues. This peptide has been identified as possessing strong antibacterial activity and low toxicity against eukaryotic cells, and it has been claimed that some types of the cecropin family of peptides are capable of killing cancer cells. In this study, the host effect of cloning antibacterial peptide cecropinB2 was investigated. Three different host expression systems were chosen, i.e., Escherichia coli, Bacillus subtilis and Pichia pastoris. Two gene constructs, cecropinB2 (cecB2) and intein-cecropinB2 (INT-cecB2), were applied. Signal peptide and propeptide from Armigeres subalbatus were also attached to the gene construct. The results showed that the best host for cloning cecropinB2 was P. pastoris SMD1168 harboring the gene of pGAPzαC-prepro-cecB2 via Western blot confirmation. The cecropinB2 that was purified using immobilized-metal affinity chromatography resin showed strong antibacterial activity against the Gram-negative strains, including the multi-drug-resistant bacteria Acinetobacter baumannii.

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Citations

Nov 25, 2018·Biotechnology and Applied Biochemistry·Yi-Ting FangYung-Chuan Liu
Feb 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Ting FangYung-Chuan Liu

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Datasets Mentioned

BETA
SMD1168

Methods Mentioned

BETA
PCR
electrophoresis
protein assay

Software Mentioned

imageJ

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