Antibacterial photodynamic treatment of periodontopathogenic bacteria with indocyanine green and near-infrared laser light enhanced by Trolox(TM)

Lasers in Surgery and Medicine
Stefan KranzBernd W Sigusch

Abstract

It has been shown that certain vitamins can significantly enhance the effect of photodynamic anti-tumor therapy. Unfortunately, there is no sufficient information available about the impact of those antioxidants on antimicrobial Photodynamic Therapy (aPDT). The present study is aimed at investigating the antimicrobial effect of the dye indocyanine green (ICG) in the presence of Trolox(TM) , a vitamin E analogue, upon irradiation with near-infrared (NIR) laser light (808 nm) on the gramnegative periodontopathogenic bacteria Aggregatibacter actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.) and Fusobacterium nucleatum (F.n.). Bacteria solved in PBS were incubated with ICG (50-500 μg/ml) in the presence and absence of Trolox(TM) (2 mM). Irradiation was performed after 10 minutes of dark-incubation with NIR-laser-light (25-100 J/cm(2) , 810 nm). During treatment, temperature was also recorded inside the bacterial solutions. The treated suspensions were serial diluted and plated onto blood agar plates. After anaerobe cultivation for 5 days the colony-forming units (CFU/ml) were determined. The antibacterial effect was ICG-concentration and exposure dependent. It was found that high ICG-concentrations and light fluence rat...Continue Reading

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Citations

May 19, 2017·Journal of Biophotonics·Ilaiáli S LeiteNatalia M Inada
Jan 19, 2017·International Journal of Molecular Sciences·Yasuyuki SasakiMitsuo Fukuda
May 2, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Carlos Bermúdez-JiménezGabriel Martínez-Castañón
Jul 13, 2018·Dentistry Journal·Islam A Abdelaziz Ali, Prasanna Neelakantan
Oct 13, 2020·Photodermatology, Photoimmunology & Photomedicine·Mohammad MotamedifarMahtab Hadadi

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