Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

Frontiers in Microbiology
Lilit TonoyanVincent O'Flaherty

Abstract

Antibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable salts-iodide and thiocyanate-in the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillin-resistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.8-31.3 and 31.3-250 μg ml-1, respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, ...Continue Reading

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Citations

Jun 10, 2018·Journal of Biophotonics·Liyi HuangMichael R Hamblin
Jan 7, 2021·Biomacromolecules·Davide CalifanoKaren J Edler
Oct 29, 2020·Communications Biology·Brell JenniferJohannes Stöckl
Nov 4, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V SalminA B Salmina

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Methods Mentioned

BETA
electrophoresis
fluorescence assay

Software Mentioned

QCapture Pro

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