Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm

Clinical Oral Investigations
Maria T P AlbuquerqueMarco C Bottino

Abstract

This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. Dentin specimens (4 × 4 × 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5% level. Both the TAP-mimic sca...Continue Reading

References

Apr 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·S TanaseS Yoshida
Apr 20, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·I N RôçasA M Coelho
Apr 22, 2008·International Endodontic Journal·L M SassoneM Feres
Jan 27, 2009·Journal of Endodontics·Ling-Huey ChuehChun-Pin Chiang
Jul 21, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·B Tugba TurkTansel Ozturk
Aug 26, 2009·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Akhil Chandra
Mar 12, 2013·Journal of Tissue Engineering and Regenerative Medicine·Marco C BottinoAna H A Bressiani
Sep 24, 2013·Journal of Dental Research·M C BottinoR L Gregory
Mar 29, 2014·Journal of Endodontics·Riyadh I AlthumairyAnibal Diogenes
Apr 5, 2014·Journal of Endodontics·Anibal R DiogenesKenneth M Hargreaves
Apr 29, 2014·Journal of Endodontics·Juliana Yuri NagataAdriana de Jesus Soares
Sep 10, 2014·Journal of Endodontics·Jadesada PalasukMarco C Bottino
Sep 10, 2014·Journal of Dental Research·M T P AlbuquerqueM C Bottino
Oct 6, 2014·Journal of Endodontics·Domenico RicucciIsabela N Rôças
Oct 11, 2014·Journal of Endodontics·Evangelos G KontakiotisAnastasia Agrafioti
Feb 24, 2015·Journal of Endodontics·Maria T P AlbuquerqueMarco C Bottino
Apr 29, 2015·Journal of Endodontics·Maria T P AlbuquerqueMarco C Bottino
Jun 14, 2015·Journal of Endodontics·Yu WangChengfei Zhang

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Citations

Oct 4, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marco C BottinoPaul C Edwards
Oct 17, 2017·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·David HwangAvina Paranjpe
May 14, 2020·Expert Opinion on Drug Delivery·Maurício G C SousaOctávio L Franco
Jun 16, 2018·Materials·Manuel Toledano-OsorioManuel Toledano
Sep 21, 2020·Journal of Endodontics·Juliana S RibeiroMarco C Bottino

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