Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa

Frontiers in Microbiology
Shimei WuChaomin Sun

Abstract

Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors, thus leading to major problems in many fields, such as clinical infection, food contamination, and marine biofouling. In this study, we report the purification and characterization of an exopolysaccharide EPS273 from the culture supernatant of marine bacterium P. stutzeri 273. The exopolysaccharide EPS273 not only effectively inhibits biofilm formation but also disperses preformed biofilm of P. aeruginosa PAO1. High performance liquid chromatography traces of the hydrolyzed polysaccharides shows that EPS273 primarily consists of glucosamine, rhamnose, glucose and mannose. Further investigation demonstrates that EPS273 reduces the production of the virulence factors pyocyanin, exoprotease, and rhamnolipid, and the virulence of P. aeruginosa PAO1 to human lung cells A549 and zebrafish embryos is also obviously attenuated by EPS273. In addition, EPS273 also greatly reduces the production of hydrogen peroxide (H2O2) and extracellular DNA (eDNA), which are important factors for biofilm formation. Furthermore, EPS273 exhibits strong antioxidant potential by quenching hydroxyl and superoxide anion radicals. Notably, the anti...Continue Reading

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Citations

Apr 9, 2017·Applied Biochemistry and Biotechnology·Mervat Morsy Abbas Ahmed El-GendyAhmed M A El-Bondkly
Mar 17, 2020·Biocontrol Science·Chun Yao AngTamrin M Lal
Mar 7, 2017·Frontiers in Microbiology·Srikkanth BalasubramanianUsama R Abdelmohsen
May 24, 2020·Applied and Environmental Microbiology·Yinglong SongFang Qian
Apr 20, 2018·Frontiers in Microbiology·Perumal Karthick, Raju Mohanraju
May 20, 2020·Journal of Microbiology and Biotechnology·Jongbin ParkEun Bae Kim
Jan 23, 2018·Journal of Agricultural and Food Chemistry·Jin-Wei ZhouAi-Qun Jia
Aug 10, 2021·Colloids and Surfaces. B, Biointerfaces·Maryam TavafoghiMaria Dittrich

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Methods Mentioned

BETA
PCR
gel filtration
Assay

Software Mentioned

BLAST

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