PMID: 26323443Sep 2, 2015Paper

Antibiofilm and Antimicrobial Activity of Polyethylenimine: An Interesting Compound for Endodontic Treatment

The Journal of Contemporary Dental Practice
Joana BarrosIrene Pina-Vaz

Abstract

Bacteria levels of necrotic teeth are greatly reduced after endodontic treatment procedures but the presence of persisting microorganisms leads to continuous efforts to develop materials with antimicrobial properties. The purpose of the study was to determine the antimicrobial activity of polyethylenimine (PEI) against common bacteria and yeasts, regarding planktonic cells and biofilm, and to clarify its antimicrobial mechanism of action through flow cytometry. The antibiofilm and antimicrobial effect of PEI was determined against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Candida albicans strains using reference protocols. The effect of PEI was evaluated regarding adhesion, biofilm formation and biofilm disaggregation. In order to understand PEI cellular effects flow cytometric analysis was performed with different fluorescent markers. It was verified that minimal inhibitory concentrations (MIC) values and minimal lethal concentrations (MLC) obtained for PEI were similar and ranged between 50 and 400 mg/l, proving the microbicidal and fungicidal activity of this compound. Antibiofilm activity was also proved for all the microorganisms. Severe lesion of the membrane and cell depolarization was demonstrat...Continue Reading

References

Nov 14, 1997·Microbiology·Ilkka M HelanderPertti Koski
Jun 1, 1999·The Journal of Antimicrobial Chemotherapy·I M Gould
Dec 21, 2004·International Endodontic Journal·R S HemsD A Spratt
Jan 24, 2006·Journal of Endodontics·Charles H StuartChristopher B Owatz
Jul 5, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Miriam BreunigAchim Goepferich
Oct 22, 2008·Journal of Endodontics·José F Siqueira, Isabela N Rôças
Mar 20, 2010·Methods in Molecular Biology·Cidália Pina-Vaz, Acácio Gonçalves Rodrigues
Sep 21, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nick D WeberMaría Ángeles Muñoz-Fernández
Jan 20, 2012·Journal of Colloid and Interface Science·Ismael C BellettiniEdson Minatti

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Citations

Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Iolanda FrancoliniGianfranco Donelli
Mar 8, 2018·Macromolecular Bioscience·Lena RichterJoerg C Tiller
Aug 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hang N NguyenDebora F Rodrigues
Aug 20, 2021·Journal of Applied Biomaterials & Functional Materials·Aqsa ShahidMohsin Khurshid

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