Antibiofilm Properties of Temporin-L on Pseudomonas fluorescens in Static and In-Flow Conditions

International Journal of Molecular Sciences
Angela Di SommaAngela Duilio

Abstract

Biofilms consist of a complex microbial community adhering to biotic or abiotic surfaces and enclosed within a protein/polysaccharide self-produced matrix. The formation of this structure represents the most important adaptive mechanism that leads to antibacterial resistance, and therefore, closely connected to pathogenicity. Antimicrobial peptides (AMPs) could represent attractive candidates for the design of new antibiotics because of their specific characteristics. AMPs show a broad activity spectrum, a relative selectivity towards their targets (microbial membranes), the ability to act on both proliferative and quiescent cells, a rapid mechanism of action, and above all, a low propensity for developing resistance. This article investigates the effect at subMIC concentrations of Temporin-L (TL) on biofilm formation in Pseudomonas fluorescens (P. fluorescens) both in static and dynamic conditions, showing that TL displays antibiofilm properties. Biofilm formation in static conditions was analyzed by the Crystal Violet assay. Investigation of biofilms in dynamic conditions was performed in a commercial microfluidic device consisting of a microflow chamber to simulate real flow conditions in the human body. Biofilm morphology w...Continue Reading

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Citations

Jul 24, 2021·Journal of Medicinal Chemistry·Rosa BellavitaPaolo Grieco

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Methods Mentioned

BETA
Laser
confocal microscopy

Software Mentioned

Leica Las X
Image J
COMSTAT
MATLAB©

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