Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette

Lab on a Chip
Ladislav DerzsiPiotr Garstecki

Abstract

We demonstrate a standalone microfluidic chip that allows us to carry out commonly executed antibiotic susceptibility assays in an array of nanoliter droplets. We eliminated the need for automation in performing an exemplary complicated liquid handling assay on a chip. Operations on droplets are hard-wired into the microfluidic chip. The liquid handling protocol can be executed with a simple and commonly available source of flow such as an automatic manual pipette. The system passively prepares a series of dilutions of a chemical compound and mixes them with portions of the sample. The precision of metering, merging, mixing, and splitting of discrete portions of liquid samples is rooted in the passive capillary action in microfluidic traps and not in the precision of dosing with a pipette. We show an exemplary use of the device in the determination of the minimum inhibitory concentration (MIC) of ampicillin against E. coli ATCC 25922.

References

Apr 22, 2004·Lab on a Chip·Glenn M Walker, David J Beebe
Oct 26, 2007·Lab on a Chip·Michael W ToepkeDavid J Beebe
Sep 25, 2008·Lab on a Chip·Alison M Skelley, Joel Voldman
Oct 23, 2008·Lab on a Chip·Xize NiuAndrew J deMello
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andres W MartinezGeorge M Whitesides
Jun 25, 2009·Analytical Chemistry·Margaret Macris KissJohn H Leamon
Jul 29, 2009·Lab on a Chip·Wenbin DuRustem F Ismagilov
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeremy J AgrestiDavid A Weitz
May 7, 2010·Lab on a Chip·Jenifer Clausell-TormosChristoph A Merten
Jun 24, 2010·Angewandte Chemie·Ashleigh B ThebergeWilhelm T S Huck
Oct 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Adam R AbateDavid A Weitz
Nov 10, 2010·Lab on a Chip·Paul AbbyadCharles N Baroud
Nov 11, 2010·Lab on a Chip·Hongying ZhuAydogan Ozcan
May 24, 2011·Nature Chemistry·Xize NiuAndrew J deMello
Oct 1, 2011·Lab on a Chip·Andrew C HatchAbraham P Lee
Nov 1, 2011·Analytical Chemistry·Benjamin J HindsonBill W Colston
Dec 14, 2011·Lab on a Chip·Conrad LochovskyAxel Günther
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bachir El DebsChristoph A Merten
Jan 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rémi DanglaCharles N Baroud
Aug 24, 2013·Lab on a Chip·Piotr M KorczykPiotr Garstecki
Oct 1, 2013·Lab on a Chip·Etienne FradetCharles N Baroud
Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Philip N DuncanElliot E Hui
Jan 10, 2014·Biomicrofluidics·Volkert van SteijnPiotr Garstecki
Aug 22, 2014·Nature Chemistry·Martin FischlechnerFlorian Hollfelder

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Citations

Jul 6, 2017·The Analyst·W PostekP Garstecki
May 24, 2016·Lab on a Chip·Tomasz S KaminskiPiotr Garstecki
Oct 19, 2016·Lab on a Chip·Gabriel AmselemCharles N Baroud
Sep 1, 2017·Chemical Society Reviews·T S Kaminski, P Garstecki
Oct 23, 2019·Microsystems & Nanoengineering·Pengfei ZhangTza-Huei Wang
Nov 1, 2016·Biomedical Microdevices·Jennifer CampbellAlexis F Sauer-Budge
Feb 6, 2017·Scientific Reports·Swastika S Bithi, Siva A Vanapalli
Jul 26, 2018·Frontiers in Microbiology·Marta Veses-GarciaAgneta Richter-Dahlfors
Aug 7, 2017·Technology·Fatemeh JalaliDaniel Irimia
Aug 20, 2019·Biosensors & Bioelectronics·Bhagaban BeheraHardik J Pandya
Dec 30, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Priyanka SharanJuliane Simmchen

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