Antibiotic Activity of a Paraphaeosphaeria sporulosa -Produced Diketopiperazine against Salmonella enterica

Journal of Fungi
Raffaele CarrieriFrancesco Vinale

Abstract

A diketopiperazine has been purified from a culture filtrate of the endophytic fungus Paraphaeosphaeria sporulosa, isolated from healthy tissues of strawberry plants in a survey of microbes as sources of anti-bacterial metabolites. Its structure has been determined by nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analyses and was found to be identical to cyclo(L-Pro-L-Phe) purified from species of other fungal genera. This secondary metabolite has been selected following bioguided-assay fractionation against two strains of Salmonella enterica, the causal agent of bovine gastroenteritis. The diketopiperazine cyclo(L-Pro-L-Phe), isolated for the first time from Paraphaeosphaeria species, showed minimum inhibitory concentration (MIC) values of 71.3 and 78.6 μg/mL against the two S.enterica strains. This finding may be significant in limiting the use of synthetic antibiotics in animal husbandry and reducing the emergence of bacterial multidrug resistance. Further in vivo experiments of P. sporulosa diketopiperazines are important for the future application of these metabolites.

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Citations

Apr 2, 2021·Frontiers in Bioengineering and Biotechnology·Rosa SagitaKristina Haslinger

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Datasets Mentioned

BETA
MN017716
MN017716.1

Methods Mentioned

BETA
Assay
column chromatography
nuclear magnetic resonance

Software Mentioned

molecular evolutionary genetics analysis ( MEGA )
drc
BLAST
tidyverse
R environment
Agilent MassHunter Data
MassHunter Qualitative Analysis
BLASTn

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