PMID: 375969May 29, 1979Paper

Antibiotic effects on the photoinduced affinity labeling of Escherichia coli ribosomes by puromycin

Biochemistry
P G GrantB S Cooperman

Abstract

The effect of ribosomal antibiotics on the photoinduced affinity labeling of Escherichia coli ribosomes by puromycin [Cooperman, B.S., Jaynes, E.N., Brunswick, D.J., & Luddy, M.A. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 1974; Jaynes, E.N. Jr., Grant, P.G., Giangrande, G., Wieder, R., & Cooperman, B.S. (1978) Biochemistry 17, 561] has been studied. Although blasticidin S, sparsomycin, lincomycin, and erythromycin are essentially without effect, major changes are seen on addition of either chloramphenicol or tetracycline. The products of photoincorporation have been characterized by one- and two-dimensional gel electrophoresis and by specific immunoprecipitation with antibodies to ribosomal proteins. In the presence of chloramphenicol, protein S14 becomes the major labeled protein. In the presence of tetracycline, L23 remains the major labeled protein, but the yield of labeled ribosomes is enormously increased, and the labeling is more specific for L23. These results are discussed in terms of the known modes of action of these antibiotics and the photoreactivity of tetracycline.

References

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Citations

Jan 1, 1986·Progress in Biophysics and Molecular Biology·K Nagano, M Harel
Jan 1, 1988·Progress in Biophysics and Molecular Biology·H A RauéW Musters
Jan 1, 1987·Pharmacology & Therapeutics·B S Cooperman
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M OlsonB S Cooperman
May 1, 1980·European Journal of Biochemistry·F Le GofficD Bonnet
Aug 25, 1980·FEBS Letters·R A GoldmanT R Tritton
Jun 13, 1983·FEBS Letters·A S Spirin
Jan 1, 1980·Annals of the New York Academy of Sciences·B S Cooperman
Sep 17, 1988·Journal of Theoretical Biology·K NaganoM Takezawa
Sep 1, 1981·Biochemistry·A M ReboudJ P Reboud
Apr 23, 1985·Biochemistry·C Weitzmann, B S Cooperman
Dec 28, 1984·Journal of Chromatography·A R KerlavageB S Cooperman

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