Jul 17, 2014

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study

Journal of Materials Science. Materials in Medicine
M GhadiriR Rohanizadeh

Abstract

Different materials in form of sponge, hydrogel and film have been developed and formulated for treating and dressing burn wounds. In this study, the potential of Laponite, a gel forming clay, in combination with an antimicrobial agent (mafenide), as a wound dressing material was tested in vitro. Laponite/mafenide (Lap/Maf) hydrogel was formulated in three different ratios of Lap/Maf 1:1, 1:2, 1:3. Laponite/mafenide/alginate (Lap/Maf/Alg) film was also formulated by combining Lap/Maf gel (1:1) with alginate. Intercalation rate of mafenide into the layers of Laponite nanoparticles and physico-chemical properties, including wound dressing characteristics of materials were studied using various analytical methods. Furthermore, the degradation of materials and the release profile of mafenide were investigated in simulated wound exudates fluid and antibacterial effectiveness of the eluted mafenide was tested on a range of bacterial species. The cytotoxicity of materials was also evaluated in skin fibroblast culture. The results showed that mafenide molecules were intercalated between the nano-sized layers of Laponite. The eluted mafenide showed active antibacterial effects against all three tested bacteria. All intercalated mafenide...Continue Reading

  • References20
  • Citations13

References

  • References20
  • Citations13

Citations

Mentioned in this Paper

Alginate
Skin Fibroblast
Bacteriocidal Agents
Cytotoxicity
Bacterial Physiological Phenomena
Hydrogel
Exudate
Observation Method - Cytotoxicity
Fibroblasts
Wound Healing

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