Antibiotic-induced oligomerisation of group I intron RNA

Journal of Molecular Biology
H Wank, R Schroeder

Abstract

Antibiotics act as inhibitors of various biological processes. Here we demonstrate that some tuberactinomycins, hitherto known as inhibitors of prokaryotic protein synthesis and of group I intron self-splicing, have a modulatory effect on group I intron RNAs. The linear intron, which is excised during the self-splicing process, is still an active molecular capable of performing an intramolecular transesterification resulting in a circular molecule. However, in the presence of sub-inhibitory concentrations of tuberactinomycins, the intron reacts intermolecularly leading to the formation of linear head-to-tail intron-oligomers. The antibiotic stimulates the intron to react in trans instead of in cis. The phage T4-derived td intron uses the same sites for oligomerisation as for circularisation. Gel- retardation experiments demonstrate that the intron RNA forms non-covalent complexes in the presence of the antibiotic. It might be envisaged that the role of these peptide antibiotics is to bridge RNA molecules mediating RNA-RNA interactions and thus enabling their reaction. The tuberactinomycins are further able to induce the interaction of heterologous introns. The ligation of the T4 phage-derived td intron with the Tetrahymena rRNA...Continue Reading

Citations

Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·R H GriffeyS T Crooke
Jan 6, 2009·Journal of Biochemistry·Yoshiya IkawaTan Inoue
Jun 7, 2005·Chembiochem : a European Journal of Chemical Biology·Richard SzilaghiThomas Weimar
Nov 14, 2007·Molecular Biotechnology·Shao-Yao YingShi-Lung Lin
Jul 19, 2008·IUBMB Life·Anastassios VourekasDenis Drainas
Jan 1, 1997·Progress in Biophysics and Molecular Biology·M G Wallis, R Schroeder

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