Antibiotic resistance gene transfer between Streptococcus gordonii and Enterococcus faecalis in root canals of teeth ex vivo

Journal of Endodontics
Christine M SedgleySusan E Flannagan

Abstract

Multiple bacterial species coexisting in infected root canals might interact, but evidence for interspecies gene transfer is lacking. This study tested the hypothesis that horizontal exchange of antibiotic resistance can occur between different bacterial species in root canals. Transfer of the conjugative plasmid pAM81 carrying erythromycin resistance between 2 endodontic infection-associated species, Streptococcus gordonii and Enterococcus faecalis, was investigated in an ex vivo tooth model. Equal numbers of each species (one with pAM81 and the other plasmid-free) were combined in prepared root canals of sterilized teeth and incubated at 37 degrees C. At 24 and 72 hours, bidirectional interspecies antibiotic resistance gene transfer was evident in microorganisms recovered from teeth; average transfer frequencies from S. gordonii to E. faecalis were 10(-3) transconjugants per donor and from E. faecalis to S. gordonii were 10(-6) and 10(-7) transconjugants per donor at 24 and 72 hours, respectively. Microbial accumulations were observed on root canal walls with scanning electron microscopy. Horizontal genetic exchange in endodontic infections might facilitate adoption of an optimal genetic profile for survival.

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Citations

Sep 20, 2011·Journal of Endodontics·Gretchen B JungermannAshraf F Fouad
Sep 2, 2009·Journal of Endodontics·Antonio Rodriguez-NúñezJuan José Segura-Egea
Jan 13, 2011·FEMS Microbiology Reviews·Elizabeth Skippington, Mark A Ragan
Jul 16, 2013·Journal of Dentistry·Renata Ximenes LinsRivail Antonio Sergio Fidel
Jan 18, 2017·Journal of Dental Research, Dental Clinics, Dental Prospects·Ali KangarlouOmid Dianat
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Oct 18, 2018·Molecular Oral Microbiology·Tao GongYuqing Li
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Mar 3, 2021·Journal of Periodontology·Laís Christina P EspíndolaAna Paula V Colombo
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