Oct 30, 2013

Antibiotic resistance genes in the bacteriophage DNA fraction of human fecal samples

Antimicrobial Agents and Chemotherapy
Pablo QuirósMaite Muniesa

Abstract

A group of antibiotic resistance genes (ARGs) (blaTEM, blaCTX-M-1, mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. blaTEM, qnrA, and, blaCTX-M-1 were the most abundant, and armA, qnrS, and mecA were less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintestinal environments.

  • References61
  • Citations35

References

  • References61
  • Citations35

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Antibiotic Resistance, Microbial
DNA, Viral
Bacteriophages
Arma
Feces
5'-N-methylcarboxamideadenosine
SERPINA2P gene
Antibiotics
resistance genes

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