Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Kateřina SvobodováČeněk Novotný

Abstract

Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, blaTEM, ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100 μg/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.

References

Jan 11, 2008·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·H-D EmborgF M Aarestrup
Apr 30, 2008·Antimicrobial Agents and Chemotherapy·Joanna E Mroczkowska, Miriam Barlow
Jan 23, 2014·The Science of the Total Environment·Björn BerglundPer-Eric Lindgren
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Kateřina SvobodováČeněk Novotný
Aug 30, 2017·The Science of the Total Environment·Elizabeth Luby RiekeMichelle L Soupir

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Datasets Mentioned

BETA
MG066491
MG066492
MG066493
MG066495
MG066496
MG066497

Methods Mentioned

BETA
chemical treatment
PCR
electrophoresis
PCA

Software Mentioned

BLASTn
GraphPad
Prism
BioNumerics
ClustalW

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