Antibiotic resistance in Streptomyces lividans: fluorescence assay for streptogramin B lyase

Analytical Biochemistry
K P BatemanN W Ross

Abstract

A fluorescence assay for streptogramin B lyase, an enzyme that confers resistance to streptogramin B antibiotics, has been developed. The antibiotic substrates are fluorescent and the linear peptide products formed in the lyase-catalyzed reaction are relatively nonfluorescent. The assay has potential for assessing bacterial resistance to streptogramin B antibiotics and will be utilized to direct the purification of streptogramin B lyase from bacterial extracts.

References

Oct 2, 1990·Biochemistry·M Di GiambattistaC Cocito
Nov 6, 1990·The American Journal of Cardiology·P Constantinides
Nov 3, 1995·Science·R F Service

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