Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK.

Genes
Elissavet NikolaouAdam P Roberts

Abstract

Pneumonia is the sixth largest cause of death in the UK. It is usually caused by Streptococcus pneumoniae, which healthy individuals can carry in their nose without symptoms of disease. Antimicrobial resistance further increases mortality and morbidity associated with pneumococcal infection, although few studies have analysed resistance in naturally circulating pneumococcal isolates in adult populations. Here, we report on the resistome and associated mobile genetic elements within circulating pneumococcus isolated from adult volunteers enrolled in the experimental human pneumococcal colonisation (EHPC) research program at the Liverpool School of Tropical Medicine, UK. Pneumococcal isolates collected from 30 healthy asymptomatic adults who had volunteered to take part in clinical research were screened for antibiotic susceptibility to erythromycin and tetracycline, and whole-genome sequenced. The genetic context of resistance to one or both antibiotics in four isolates was characterised bioinformatically, and any association of the resistance genes with mobile genetic elements was determined. Tetracycline and macrolide resistance genes [tet(M), erm(B), mef(A), msr(D)] were detected on known Tn916-like integrative and conjugativ...Continue Reading

References

Mar 1, 1995·Antimicrobial Agents and Chemotherapy·B Weisblum
Oct 23, 1997·Antimicrobial Agents and Chemotherapy·A Tait-KamradtJ Sutcliffe
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Jun 12, 2001·Methods in Enzymology·A P RobertsM Wilson
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Thad B StantonHarry J Flint
Jul 5, 2005·The Journal of Infectious Diseases·David GoldblattElizabeth Miller
Dec 21, 2006·Antimicrobial Agents and Chemotherapy·Andrea J PattersonKaren P Scott
Sep 15, 2007·The Journal of Antimicrobial Chemotherapy·Philip J WarburtonWilliam G Wade
Apr 24, 2009·Journal of Clinical Microbiology·Silvio D BruggerKathrin Mühlemann
May 26, 2009·Trends in Microbiology·Adam P Roberts, Peter Mullany
Jun 13, 2009·PLoS Pathogens·Daniel M WeinbergerMarc Lipsitch
Sep 15, 2009·Lancet·Katherine L O'BrienUNKNOWN Hib and Pneumococcal Global Burden of Disease Study Team
Oct 23, 2010·Journal of Medical Microbiology·R A GladstoneS C Clarke
Feb 15, 2012·Vaccine·Jae-Hoon SongBernard Fritzell
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Feb 2, 2013·American Journal of Respiratory and Critical Care Medicine·Daniela M FerreiraStephen B Gordon
Feb 27, 2013·Journal of Visualized Experiments : JoVE·Jenna F GritzfeldStephen B Gordon
Mar 20, 2014·Bioinformatics·Torsten Seemann
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Jun 13, 2015·Scientific Reports·Elina NumminenJukka Corander
Mar 17, 2016·Scientific Reports·Sophie S AbbyEduardo P C Rocha
Aug 6, 2016·The Journal of Antimicrobial Chemotherapy·Philip J WarburtonAdam P Roberts
Oct 7, 2016·Frontiers in Cellular and Infection Microbiology·Max R Schroeder, David S Stephens
Nov 9, 2018·Nucleic Acids Research·Meng LiuHong-Yu Ou
May 21, 2019·Frontiers in Microbiology·Max R SchroederDavid S Stephens
May 23, 2019·Journal of Medical Microbiology·Fatma Nur Akdoğan KittanaKadir Serdar Diker
Oct 19, 2019·Mobile DNA·Supathep TansirichaiyaAdam P Roberts

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY898750.1
LK020687.1
AB426620.1
MT489699
JABAHC010000004.1
CP031971.1
and
CP022537.1
CAAVIC010000000

Methods Mentioned

BETA
ICE
ICEs

Software Mentioned

ResFinder
PlasmidFinder
CONJscan
SPAdes
ICEfinder
PROKKA
SNAPGENE

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Related Papers

Current Opinion in Pulmonary Medicine
Catia CillónizAntoni Torres
Expert Review of Anti-infective Therapy
Xianding DengDylan R Pillai
Chang Gung Medical Journal
Chen-Fang Ho, Tzou-Yien Lin
© 2022 Meta ULC. All rights reserved