Mar 6, 2009

Antibiotic resistance mechanisms of Escherichia coli Isolates from urinary specimens

The Korean journal of laboratory medicine
Sungwook SongSeok Hoon Jeong

Abstract

This study was designed to characterize urinary isolates of Escherichia coli that produce extended-spectrum beta-lactamases (ESBLs) and to determine the prevalence of other antimicrobial resistance genes. A total of 264 non-duplicate clinical isolates of E. coli were recovered from urine specimens in a tertiary-care hospital in Busan in 2005. Antimicrobial susceptibility was determined by disk diffusion and agar dilution methods, ESBL production was confirmed using the double-disk synergy (DDS) test, and antimicrobial resistance genes were detected by direct sequencing of PCR amplification products. E. coli isolates were classified into four phylogenetic biotypes according to the presence of chuA, yjaA, and TSPE4. DDS testing detected ESBLs in 27 (10.2%) of the 264 isolates. The most common type of ESBL was CTX-M-15 (N=14), followed by CTX-M-3 (N=8) and CTX-M-14 (N=6). All of the ESBL-producing isolates were resistant to ciprofloxacin. PCR experiments detected genes encoding DHA-1 and CMY-10 AmpC beta-lactamases in one and two isolates, respectively. Also isolated were 5 isolates harboring 16S rRNA methylases, 2 isolates harboring Qnr, and 19 isolates harboring AAC(6')-Ib-cr. Most ESBL-producing isolates clustered within phylog...Continue Reading

  • References28
  • Citations10

References

  • References28
  • Citations10

Citations

Mentioned in this Paper

Urine
Bacterial Proteins
M15 protein
Alkalescens-Dispar Group
Enzymes, antithrombotic
Ciprofloxacin
Gene Amplification Technique
16S rRNA - m7G methyltransferase
Phylogeny
Beta-Lactamase

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