Antibiotic stability over six weeks in aqueous solution at body temperature with and without heat treatment that mimics the curing of bone cement

Bone & Joint Research
E SamaraP Wahl

Abstract

Thermal stability is a key property in determining the suitability of an antibiotic agent for local application in the treatment of orthopaedic infections. Despite the fact that long-term therapy is a stated goal of novel local delivery carriers, data describing thermal stability over a long period are scarce, and studies that avoid interference from specific carrier materials are absent from the orthopaedic literature. In this study, a total of 38 frequently used antibiotic agents were maintained at 37°C in saline solution, and degradation and antibacterial activity assessed over six weeks. The impact of an initial supplementary heat exposure mimicking exothermically curing bone cement was also tested as this material is commonly used as a local delivery vehicle. Antibiotic degradation was assessed by liquid chromatography coupled to mass spectrometry, or by immunoassays, as appropriate. Antibacterial activity over time was determined by the Kirby-Bauer disk diffusion assay. The heat exposure mimicking curing bone cement had minimal effect on stability for most antibiotics, except for gentamicin which experienced approximately 25% degradation as measured by immunoassay. Beta-lactam antibiotics were found to degrade quite rapid...Continue Reading

References

Jul 6, 2000·Drugs·K Kanellakopoulou, E J Giamarellos-Bourboulis
Dec 16, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P D HoltomB Frenkel
Mar 2, 2002·The Lancet Infectious Diseases·D StengelF Porzsolt
May 31, 2003·The Journal of Antimicrobial Chemotherapy·Jane FinlayJames A Poupard
Mar 12, 2004·The Journal of Antimicrobial Chemotherapy·Jane W A Vella-BrincatStephen T Chambers
Nov 24, 2004·Clinical Orthopaedics and Related Research·Arlen D Hanssen, Mark J Spangehl
Oct 20, 2005·Clinical Pharmacokinetics·Amparo Sánchez Navarro
Jun 22, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Pang-Hsin HsiehChun-Hsiung Shih
Dec 11, 2007·The Journal of Antimicrobial Chemotherapy·Johan W MoutonOtto Cars
Mar 11, 2008·International Journal of Antimicrobial Agents·Irene GalaniHelen Giamarellou
Mar 15, 2008·The Journal of Antimicrobial Chemotherapy·Boyan BonevJudicaël Parisot
Jan 16, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Konstantinos Anagnostakos, Jens Kelm
Mar 11, 2009·Clinical Pharmacokinetics·Cornelia B LandersdorferFritz Sörgel
Feb 24, 2010·The Journal of Antimicrobial Chemotherapy·Karine BerthoinPaul M Tulkens
Oct 13, 2010·Archives of Orthopaedic and Trauma Surgery·Peter WahlThierry Buclin
May 14, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christopher R RathboneJoseph C Wenke
Aug 25, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D IarikovS Nambiar
Dec 12, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Douglas R OsmonUNKNOWN Infectious Diseases Society of America
Dec 12, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Douglas R OsmonUNKNOWN Infectious Diseases Society of America
Dec 29, 2012·Scandinavian Journal of Infectious Diseases·Håkan HanbergerUNKNOWN Swedish Reference Group for Antibiotics
Jan 11, 2013·The Journal of Antimicrobial Chemotherapy·Violeta RaverdyPaul M Tulkens
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E MatuschekG Kahlmeter
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Johannes ZanderMichael Vogeser
Jun 3, 2016·Cold Spring Harbor Perspectives in Medicine·Kevin M KrauseLynn E Connolly

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Citations

Jan 25, 2018·Bone & Joint Research·A SalehC A Higuera
Dec 26, 2018·Bone & Joint Research·B G PijlsR G H H Nelissen
Jan 29, 2020·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Bart G PijlsRob G H H Nelissen
Mar 4, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Smita GopinathAkiko Iwasaki
Jul 21, 2020·PloS One·Rebecca BrouwersRosalind J Allen
Oct 10, 2018·Scientific Reports·Belén OlivaresPaola R Campodonico
Sep 22, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dan LiVincent M Rotello
Aug 30, 2019·Journal of Orthopaedic Trauma·Willem-Jan MetsemakersUNKNOWN Fracture-Related Infection (FRI) consensus group
May 21, 2020·Bone & Joint Research·Bart G PijlsRob G H H Nelissen
Sep 23, 2020·Chinese Medical Journal·Yi-Ming XuXi-Sheng Weng
Jan 7, 2021·Biomedicines·Victoria WallPhong A Tran
Apr 16, 2021·The Journal of Bone and Joint Surgery. American Volume·Ashley E LevackDavid S Wellman
Aug 26, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M DepypereW-J Metsemakers
Jul 1, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ashley E LevackAlberto V Carli
Aug 14, 2021·NPJ Biofilms and Microbiomes·Qi WangDavid E Greenberg
Aug 10, 2019·ACS Infectious Diseases·Azucena Gonzalez GomezZeinab Hosseinidoust

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Methods Mentioned

BETA
thermal treatment

Software Mentioned

Prism
GraphPad

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