PMID: 9450523Feb 5, 1998Paper

Antibiotic susceptibility testing for Chlamydia trachomatis using flow cytometry

Cytometry
Sophie C Dessus-BabusB de Barbeyrac

Abstract

The aim of this study was to compare the effectiveness of two methods of antibiotic susceptibility testing performed on Chlamydia trachomatis-infected cells: a flow cytometric detection method and the standard method, which consists of a microscopic reading of minimal inhibitory concentration (MIC). The L2 reference strain and 13 clinical strains isolated from six patients presenting recurrent infections were tested. McCoy cells infected with an inoculum of 10(5) inclusion forming units (IFU)/ml were incubated with serial dilutions of doxycycline, ofloxacin, and erythromycin. Mean fluorescence intensity (MFI) of cells was determined by flow cytometry after staining of chlamydial inclusions with an anti-Chlamydia fluorescent monoclonal antibody. The end-point values determined by flow cytometry and microscopic reading were equivalent but presented the same imprecision. Calculation of the inhibitory concentration 50 (IC50) by flow cytometry, defined as the antibiotic concentration required to reduce the drug-free control MFI by 50%, allowed a more objective and precise evaluation of antibiotic activity than MIC. Moreover, IC50 values were reproducible, independent of the antibiotic dilution series tested, and could be used to com...Continue Reading

Citations

Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·M Maurin, D Raoult
Oct 16, 2007·The Science of the Total Environment·Mitchell S Kostich, James M Lazorchak
Oct 3, 2014·BioMed Research International·Verónica Ambriz-AviñaMario Pedraza-Reyes
Jul 31, 2013·Orvosi hetilap·Eva Pállinger
Feb 14, 2007·Journal of Korean Medical Science·Mi-Jeong KimJae-Seung Kang
May 18, 2016·Yonsei Medical Journal·Sun Myoung LeeJin Soo Lee
May 10, 2001·Microbiology and Immunology·M AmjadM R Karim

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