Antibiotic tigecycline inhibits cell proliferation, migration and invasion via down-regulating CCNE2 in pancreatic ductal adenocarcinoma.

Journal of Cellular and Molecular Medicine
Jie YangHongjuan Cui

Abstract

Recently, many researches have reported that antibiotic tigecycline has significant effect on cancer treatment. However, biomedical functions and molecular mechanisms of tigecycline in human pancreatic ductal adenocarcinoma (PDAC) remain unclear. In the current study, we tried to assess the effect of tigecycline in PDAC cells. AsPC-1 and HPAC cells were treated with indicated concentrations of tigecycline for indicated time, and then, MTT, BrdU and soft agar assay were used to test cell proliferation. The effect of tigecycline on cell cycle and cellular apoptosis was tested by cytometry. Migration and invasion were detected by wound healing assay and transwell migration/invasion assay. Expressions of cell cycle-related and migration/invasion-related protein were determined by using Western blot. The results revealed that tigecycline observably suppressed cell proliferation by inducing cell cycle arrest at G0/G1 phase and blocked cell migration/invasion via holding back the epithelial-mesenchymal transition (EMT) process in PDAC. In addition, tigecycline also remarkably blocked tumorigenecity in vivo. Furthermore, the effects of tigecycline alone or combined with gemcitabine in vitro or on PDAC xenografts were also performed. Th...Continue Reading

References

Nov 14, 2000·The American Journal of Pathology·H OuyangM S Tsao
Oct 22, 2005·Nature Reviews. Drug Discovery·Richard WenzelPeter Kirkpatrick
May 26, 2006·Antimicrobial Agents and Chemotherapy·Matthew W OlsonPatricia A Bradford
Jun 3, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anieta M SieuwertsJohn A Foekens
Dec 25, 2008·Neoplasia : an International Journal for Oncology Research·Zhong WuDan Theodorescu
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jan 15, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Peipei LiFaguang Jin
Mar 4, 2014·Biochemical and Biophysical Research Communications·Chunling TangHongjuan Cui
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Mar 26, 2015·World Journal of Gastroenterology : WJG·Daniel AnsariRoland Andersson
Dec 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaoxia ZhongHongjuan Cui
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Jan 30, 2016·Molecular Cancer Therapeutics·Rui YangHongjuan Cui
Mar 25, 2016·Fundamental & Clinical Pharmacology·Xuefeng JiaJunbo Jiao
Aug 30, 2016·The Journal of Clinical Investigation·Robert A JonesEldad Zacksenhaus
Dec 21, 2016·Frontiers in Pharmacology·Zhijie XuZhicheng Gong
May 13, 2017·Current Medicinal Chemistry·Harald ManggeJohannes Haybaeck
Jun 25, 2017·Biochemical and Biophysical Research Communications·Bo WangXiaobin Yao
Sep 6, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S J VandecasteeleR van Coster
Dec 8, 2017·Turkish Journal of Haematology : Official Journal of Turkish Society of Haematology·Fulya Yılmaz DuranEmre Mehmet Şen
Jan 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yan GengPiotr Sicinski
Apr 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Feng WangHaifa Shen
Apr 25, 2018·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J J Luke, S K Pal
Jul 4, 2018·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Shota MyojinYuho Horikoshi
Aug 18, 2018·Cell Chemical Biology·David A DouganKürşad Turgay
Jul 25, 2019·International Journal of Molecular Sciences·Zhen DongHongjuan Cui

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Citations

Jan 8, 2021·Journal of Orthopaedic Surgery and Research·Baojin YaoDaqing Zhao
Apr 12, 2021·Journal of Experimental & Clinical Cancer Research : CR·Changhong LiHongjuan Cui
May 6, 2021·International Journal of Molecular Sciences·Susmita BarmanPunita Dhawan

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Methods Mentioned

BETA
flow cytometry
protein assay
electrophoresis
PCR
xenograft

Software Mentioned

FlowJo
CompuSyn
GraphPad Prism

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