Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice

PloS One
Michela BistolettiCristina Giaroni

Abstract

Antibiotic use during adolescence may result in dysbiosis-induced neuronal vulnerability both in the enteric nervous system (ENS) and central nervous system (CNS) contributing to the onset of chronic gastrointestinal disorders, such as irritable bowel syndrome (IBS), showing significant psychiatric comorbidity. Intestinal microbiota alterations during adolescence influence the expression of molecular factors involved in neuronal development in both the ENS and CNS. In this study, we have evaluated the expression of brain-derived neurotrophic factor (BDNF) and its high-affinity receptor tropomyosin-related kinase B (TrkB) in juvenile mice ENS and CNS, after a 2-week antibiotic (ABX) treatment. In both mucosa and mucosa-deprived whole-wall small intestine segments of ABX-treated animals, BDNF and TrKB mRNA and protein levels significantly increased. In longitudinal muscle-myenteric plexus preparations of ABX-treated mice the percentage of myenteric neurons staining for BDNF and TrkB was significantly higher than in controls. After ABX treatment, a consistent population of BDNF- and TrkB-immunoreactive neurons costained with SP and CGRP, suggesting up-regulation of BDNF signaling in both motor and sensory myenteric neurons. BDNF a...Continue Reading

References

Jan 1, 1996·Annual Review of Neuroscience·G R Lewin, Y A Barde
Aug 31, 2002·Journal of Gastroenterology·Georg B T von BoyenJoachim Kirsch
Oct 9, 2002·Neuron·Liliana MinichielloMartin Korte
Sep 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·J R Grider, B E Piland
Oct 16, 2008·Cell and Tissue Research·Zheng-Dong QuJohn B Furness
Nov 13, 2008·Nature Reviews. Neuroscience·Tomás PausJay N Giedd
May 1, 2009·Nature Reviews. Gastroenterology & Hepatology·Sang H RheeEmeran A Mayer
Nov 26, 2009·European Journal of Pain : EJP·Jing YangYanqing Li
Jun 10, 2010·The Journal of Comparative Neurology·Michelle C Murphy, Edward A Fox
Dec 17, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C GiaroniG Frigo
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rochellys Diaz HeijtzSven Pettersson
Mar 13, 2012·Pharmacological Reviews·Anita E Autry, Lisa M Monteggia
Jun 9, 2012·Trends in Immunology·Carles Ubeda, Eric G Pamer
Sep 13, 2012·Nature Reviews. Neuroscience·John F Cryan, Timothy G Dinan
Dec 21, 2012·Nature Reviews. Neuroscience·Hyungju Park, Mu-ming Poo
Nov 28, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Rachel D MoloneyJohn F Cryan
Jun 25, 2014·Trends in Molecular Medicine·Yuliya E BorreJohn F Cryan
Mar 3, 2015·The Journal of Clinical Investigation·Panagiotis S Kabouridis, Vassilis Pachnis
Apr 1, 2015·Molecular and Cellular Neurosciences·Paola BrunIgnazio Castagliuolo
Apr 14, 2015·Brain, Behavior, and Immunity·Lieve DesbonnetJohn F Cryan
May 9, 2015·Autonomic Neuroscience : Basic & Clinical·Cristina Giaroni
May 13, 2015·Digestive Diseases and Sciences·Xiaojing QuanHong Xia
Mar 18, 2016·Nursing Standard
May 10, 2016·Nature Reviews. Disease Primers·Paul EnckRobin C Spiller
May 27, 2016·Nature Reviews. Disease Primers
Jun 28, 2016·Developmental Biology·Niall P Hyland, John F Cryan
Jul 20, 2016·Journal of Neurogastroenterology and Motility·Mohammad H FarzaeiRoja Rahimi
Nov 4, 2016·Neurochemical Research·Raeesah Maqsood, Trevor W Stone

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Citations

Dec 22, 2019·Biomolecules·Maria Cristina Marrone, Roberto Coccurello
Feb 29, 2020·International Journal of Molecular Sciences·Nicola ForteLuigia Cristino
Apr 3, 2019·International Journal of Molecular Sciences·Andreina BajCristina Giaroni
Jun 25, 2020·International Journal of Tryptophan Research : IJTR·Annalisa BosiAndreina Baj
Apr 1, 2020·Frontiers in Psychiatry·Tomasz SzeligowskiPhilip W J Burnet
Jul 25, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Eleonora SolariAndrea Moriondo
Aug 11, 2020·Bioscience of Microbiota, Food and Health·Yujie ZhangFang He
Sep 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaime P P FoongJoel C Bornstein
Feb 11, 2021·International Journal of Molecular Sciences·Davide BanfiAndreina Baj
Apr 11, 2021·Brain Research Bulletin·Luana M ManossoGislaine Z Réus
Dec 15, 2020·Trends in Microbiology·Amélie JolyFilipe De Vadder
May 6, 2021·Biomolecules·Vivek Kumar SharmaMohamed M Abdel-Daim
May 18, 2021·The Journal of Clinical Investigation·Kristie B Yu, Elaine Y Hsiao

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BETA
transgenic
Fluorescence microscopy

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