Antibodies against Crandell Rees feline kidney (CRFK) cell line antigens, alpha-enolase, and annexin A2 in vaccinated and CRFK hyperinoculated cats

Journal of Veterinary Internal Medicine
J C WhittemoreMichael R Lappin

Abstract

Cats inoculated with feline herpesvirus 1, calicivirus, and panleukopenia (FVRCP) vaccines grown on the Crandell Rees feline kidney (CRFK) cell line have been shown to develop anti-CRFK antibodies. The identities of common CRFK antigens are unknown. Cats inoculated with CRFK lysates and FVRCP vaccines will develop autoantibodies measurable by Western blot immunoassay. Antigens associated with these antibodies can be isolated for further study. One CRFK hyperinoculated rabbit, 44 age-matched unvaccinated kittens purchased from a commercial vendor. Commonly recognized CRFK antigens were identified by comparison of Western blot immunoassays using sera from a hyperinoculated rabbit and kittens inoculated with CRFK lysate or 1 of 4 commercially available FVRCP vaccines. Antigens were purified from CRFK lysates and sequenced. Antigen recognition was confirmed by Western blot immunoassay and indirect ELISA for 2 proteins using sera from CRFK and FVRCP inoculated kittens. CRFK antigens 47, 40, and 38 kD in size were identified. Protein isolation and sequencing identified 3 CRFK proteins as alpha-enolase, annexin A2, and macrophage capping protein (MCP). Sera from FVRCP and CRFK inoculated cats were confirmed to recognize annexin A2 and...Continue Reading

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Citations

Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick C Y WooKwok-Yung Yuen
Jul 28, 2016·The Veterinary Clinics of North America. Small Animal Practice·Rosanne E Jepson
May 17, 2006·Veterinary Microbiology·M J Day
Sep 6, 2013·Journal of Feline Medicine and Surgery·Brice S Reynolds, Hervé P Lefebvre
Mar 8, 2016·Journal of Veterinary Internal Medicine·N C FinchJ Elliott
Nov 28, 2019·Journal of Veterinary Science·Nisakorn SongaksornKriangkrai Thongkorn
Mar 19, 2021·The Journal of Veterinary Medical Science·Manabu KoikeAki Koike

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