Antibodies against Drosophila potassium channels identify membrane proteins across species

Brain Research. Molecular Brain Research
J A BarbasA Ferrus

Abstract

Shaker is a complex locus (ShC) in Drosophila that encodes components of the K+ channel responsible for the IA current. We have raised antibodies against synthetic peptides of selected sequences from the Sh products. One of the antisera identifies a 71 kDa protein band in immunoblots from Drosophila neural membrane proteins. We demonstrate that this protein is encoded within the viable (V) region of the ShC since deletions and breakpoints in this part of the complex eliminate this band from the immunoblots. Certain Sh mutations abolish the production of this product while other do not seem to interfere with it. The same antiserum identifies bands of different apparent molecular weight (Mr) in membrane extracts of nervous systems of a variety of organisms including vertebrates.

References

May 31, 1988·Biochemical and Biophysical Research Communications·H Rehm, M Lazdunski
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L E IversonB Rudy
Aug 1, 1988·Trends in Biochemical Sciences·Y SaimiC Kung
Sep 1, 1985·Journal of Neurogenetics·M A Tanouye, A Ferrus
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M A TanouyeS C Fujita

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Citations

Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·F Mi, G A Berkowitz
Jul 1, 1994·Plant Physiology·F MiG A Berkowitz

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