PMID: 6401699Jan 1, 1983Paper

Antibodies against polyethylene glycol produced in animals by immunization with monomethoxy polyethylene glycol modified proteins

International Archives of Allergy and Applied Immunology
A W Richter, E Akerblom

Abstract

Antibodies to polyethylene glycol (PEG) were raised in rabbits by immunization with monomethoxy polyethylene glycol modified ovalbumin (OA), bovine superoxide dismutase (SOD), and ragweed pollen extract (Rag), given in Freund's complete adjuvant (FCA). Immunogenicity depended on the nature of the protein and the degree of modification. With modified OA, in the presence of FCA, the majority of animals showed an anti-PEG response. With modified SOD and Rag only a small proportion of animals responded. In the absence of FCA, modified OA, given s.c., did not elicit any anti-PEG antibody response in rabbits and only a weak response in mice. PEG of MW 10,000 and 100,000 given in FCA was found nonimmunogenic in rabbits, and PEG of MW 5.9 X 10(6), given s.c. to mice, showed no or very poor immunogenic properties. Gel diffusion, heterologous passive anaphylaxis and passive hemagglutination were used to demonstrate anti-PEG antibodies raised to PEG-modified proteins. Specificity was confirmed by hapten inhibition of precipitation, inhibition of passive hemagglutination and cross-reactivity tests. PEG of MW greater than or equal to 4,000 produced specific precipitates, smaller molecules acted as monovalent haptens. From hapten inhibition ...Continue Reading

Citations

May 16, 2013·Pharmaceutical Research·Huub SchellekensVera Brinks
Feb 15, 2001·Current Gastroenterology Reports·M L Shiffman
Jan 1, 1985·International Journal of Immunopharmacology·J TsujiI Yamamoto
Jun 8, 2002·Advanced Drug Delivery Reviews·M J RobertsJ M Harris
Sep 23, 2003·Advanced Drug Delivery Reviews·Paolo Caliceti, Francesco M Veronese
Aug 19, 2010·Chemical Research in Toxicology·Carrie A SimpsonDavid E Cliffel
Jun 30, 2007·Hybridoma·David A WunderlichHaren Vasavada
Oct 29, 2013·Annual Review of Pharmacology and Toxicology·Matthew PalomboPatrick J Sinko
Dec 18, 2013·Biological & Pharmaceutical Bulletin·Fan ZhangHai-tong Wan
Mar 19, 2014·Toxicology and Applied Pharmacology·Yosuke HashimotoHiroshi Kiwada
Jan 3, 2016·Cellular Immunology·Mathieu IngUNKNOWN ABIRISK consortium
Nov 1, 2008·Expert Opinion on Drug Discovery·Mehrdad HamidiAmir Azadi
Dec 30, 2014·Expert Opinion on Drug Delivery·Ana Tari Ashizawa, Jorge Cortes
Aug 31, 2012·Expert Opinion on Drug Delivery·Ricardo P GarayPascal Richette
Jul 12, 2012·Drug Development and Industrial Pharmacy· AjazuddinParijat Kanaujia
Jan 1, 1996·Journal of Drug Targeting·G E FrancisA K Agrawal
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng ZhangShaoyi Jiang
Sep 20, 2011·Acta Biomaterialia·Hua-Fen WangRen-Xi Zhuo
Aug 11, 2011·Journal of Pharmacological and Toxicological Methods·Yijuan LiuRay Yin
Oct 1, 2008·Drug Discovery Today. Technologies·Francesco M Veronese, Gianfranco Pasut
Jun 2, 2009·International Journal of Pharmaceutics·Xiaowei LuXirong Guo
Sep 12, 2015·Current Opinion in Chemical Biology·Yizhi Qi, Ashutosh Chilkoti
Jan 14, 2010·Biotechnology Journal·Simona JevsevarVladka Gaberc Porekar
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·M L NucciA Abuchowski
Feb 25, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Qi Yang, Samuel K Lai
Aug 13, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amr S Abu LilaTatsuhiro Ishida
Mar 3, 2012·Pharmaceutical Development and Technology·Priti TomarVinod Kumar Dixit
Apr 24, 2014·Computational and Structural Biotechnology Journal·Remko van VughtEefjan Breukink
Sep 11, 2014·Drug Discovery Today·Johan J F VerhoefHuub Schellekens
May 26, 2016·Acta Biomaterialia·Allan S Hoffman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.