Antibodies against RNA hydrolyze RNA and DNA

Journal of Molecular Recognition : JMR
M A KrasnorutskiiGeorgy A Nevinsky

Abstract

Immunization of animals with DNA leads to the production of anti-DNA antibodies (Abs) demonstrating both DNase and RNase activities. It is currently not known whether anti-RNA Abs can possess nuclease activities. In an attempt to address this question, we have shown that immunization of three rabbits with complex of RNA with methylated BSA (mBSA) stimulates production of IgGs with RNase and DNase activities belonging to IgGs, while polyclonal Abs from three non-immunized rabbits and three animals immunized with mBSA are catalytically inactive. Affinity chromatography of IgGs from the sera of autoimmune (AI) patients on DNA-cellulose usually demonstrates a number of fractions, all of which effectively hydrolyze both DNA and RNA, while rabbit catalytic IgGs were separated into Ab subfractions, some of which demonstrated only DNase activity, while others hydrolyzed RNA faster than DNA. The enzymic properties of the RNase and DNase IgGs from rabbits immunized with RNA distinguish them from all known canonical RNases and DNases and DNA- and RNA-hydrolyzing abzymes (Abzs) from patients with different AI diseases. In contrast to RNases and AI RNA-hydrolyzing Abs, rabbit RNase IgGs catalyze only the first step of the hydrolysis reactio...Continue Reading

References

Sep 6, 1976·Biochimica Et Biophysica Acta·K AkagiM Yamanaka
Aug 16, 1978·Molecular and Cellular Biochemistry·S MitsuhashiN Yamaguchi
May 1, 1992·Science·A M ShusterA G Gabibov
Mar 1, 1988·Immunology Letters·Y ShoenfeldN Gadoth
Oct 1, 1972·Cellular Immunology·A A Gottlieb, R H Schwartz
Nov 1, 1971·Archives of Biochemistry and Biophysics·W Y LeeA H Sehon
Feb 1, 1993·European Journal of Immunology·E RazD Eilat
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G V GololobovA G Gabibov
Jun 1, 1994·Journal of Molecular Recognition : JMR·D Suck
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S TawfikZ Eshhar
Jan 1, 1997·Methods : a Companion to Methods in Enzymology·T N MarionD M Tillman
Nov 27, 1998·Nature Biotechnology·M C FletcherJ S Lee
Dec 16, 1998·The Journal of Biological Chemistry·B M FisherR T Raines
Apr 24, 1999·Applied Biochemistry and Biotechnology·V N BunevaG A Nevinsky
May 29, 2000·Applied Biochemistry and Biotechnology·H Sinohara, K Matsuura
May 29, 2000·Applied Biochemistry and Biotechnology·G A NevinskyV N Buneva
Oct 16, 2002·Journal of Immunological Methods·Yoshisuke Nishi
Oct 16, 2002·Journal of Immunological Methods·Georgy A Nevinsky, Valentina N Buneva
Sep 2, 2003·Biochemistry. Biokhimii︠a︡·V N BunevaG A Nevinsky
Nov 5, 2003·Journal of Cellular and Molecular Medicine·G A Nevinsky, Valentina N Buneva
Nov 8, 2006·Molekuliarnaia biologiia·E S OdintsovaG A Nevinskiĭ

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Citations

Feb 12, 2009·International Immunology·Michael A KrasnorutskiiGeorgy A Nevinsky
Nov 16, 2011·Biochemistry. Biokhimii︠a︡·M A KrasnorutskiiG A Nevinsky
Apr 16, 2013·Biochemistry. Biokhimii︠a︡·V N BunevaG A Nevinsky
Jan 23, 2016·Journal of Molecular Recognition : JMR·Svetlana V BaranovaGeorgy A Nevinsky
Apr 13, 2010·Biochemical and Biophysical Research Communications·Hye-Ryeong JunMyung-Hee Kwon
Sep 19, 2015·Open Biology·Evgeny A ErmakovGeorgy A Nevinsky
Dec 24, 2008·Biochemical and Biophysical Research Communications·Dong-Sik KimYong-Sung Kim
Sep 16, 2010·Journal of Molecular Recognition : JMR·Irina A KostrikinaGeorgy A Nevinsky
Aug 4, 2011·Journal of Molecular Recognition : JMR·Haggag S ZeinKazutaka Miyatake
Nov 20, 2016·Journal of Molecular Recognition : JMR·Svetlana V BaranovaGeorgy A Nevinsky
Aug 6, 2020·International Journal of Molecular Sciences·Evgeny A ErmakovValentina N Buneva
Apr 2, 2010·Molecular Immunology·Haggag S ZeinKazutaka Miyatake

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