Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis

Arthritis Research & Therapy
Andrew D CookMerrill J Rowley

Abstract

Antibodies against intact type II collagen (CII) are a feature of rheumatoid arthritis (RA) but have limited diagnostic value. Here we assess whether either of the two major cyanogen bromide fragments of CII, namely CB10 or CB11, are more sensitive substrates for the detection of antibodies in RA. Cleavage of bovine CII with cyanogen bromide yielded CB10 and CB11; these were purified by column chromatography for use in an enzyme-linked immunosorbent assay. Serum antibodies were measured in patients with RA, psoriatic arthritis (PsA), osteoarthritis (OA) and blood donors. Results were compared with those using intact CII. Antibodies against CB10 were found in as many as 88% of 96 patients with long-standing RA, but only 12% of 33 patients with PsA, 6% of 34 patients with OA and 3% of 93 control sera. Lower frequencies for these diseases were obtained on testing for antibodies against CB11: 50%, 6%, 21% and 2%, respectively. The sensitivity of detection in RA of antibodies against CB10 compared with antibodies against intact CII (88% versus 24%) was not at the expense of specificity, which remained high at 94%. The much higher frequency of antibodies against CB10 in RA than in other rheumatic diseases or control sera indicates th...Continue Reading

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Citations

Mar 8, 2007·Evidence-based Complementary and Alternative Medicine : ECAM·Yun-Kyoung YimJung-Eun Kim
Oct 7, 2005·Arthritis Research & Therapy·Duncan E CrombieMerrill J Rowley
Jan 27, 2007·Arthritis Research & Therapy·Kutty Selva Nandakumar, Rikard Holmdahl
Feb 19, 2011·Rheumatology International·Hideshi YamazakiShun'ichiro Taniguchi
Jun 4, 2008·Modern Rheumatology·Merrill J RowleyRikard Holmdahl
Jun 10, 2009·Cell and Tissue Research·Kutty Selva Nandakumar
Jun 1, 2008·Expert Opinion on Medical Diagnostics·Aristo Vojdani
Feb 12, 2008·Scandinavian Journal of Immunology·S OestergaardM A Karsdal
Apr 26, 2008·International Reviews of Immunology·Roberto BeiAndrea Modesti
Feb 1, 2007·Arthritis and Rheumatism·Lazaros I Sakkas, Chris D Platsoucas
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Mar 3, 2010·Immunological Reviews·Ann Duskin, Robert A Eisenberg
Jan 1, 2012·Scientifica·Christiane S Hampe
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Dec 15, 2012·Arthritis and Rheumatism·Allyson M CroxfordMerrill J Rowley

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Methods Mentioned

BETA
circular dichroism
enzyme-linked immunosorbent assay
ELISA

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