PMID: 9541456Apr 16, 1998Paper

Antibodies and T cells against synthetic peptides of the C-terminal domain (Hc) of botulinum neurotoxin type A and their cross-reaction with Hc

Immunology Letters
M OshimaM Z Atassi

Abstract

Seventeen peptides containing T cell and/or antibody (Ab) epitopes previously localized on Hc of botulinum neurotoxin type A were used in SJL and BALB/c mice as immunogens either individually or as an equimolar mixture of groups that contained epitopes of T cells, Abs or both, to determine their abilities to generate T cells and/or Abs that recognize intact Hc. In SJL, peptide 897-915 which included both T cell and Ab epitopes, elicited Abs that cross-reacted very strongly with Hc. In BALB/c, peptides 869-887, 883-901, 981-999 and 1275-1296 which contained Ab epitopes generated Abs that cross-reacted strongly with Hc. A mixture of peptides that contained T cell and Ab epitopes was effective in both strains in eliciting T cells and Abs that cross-reacted with Hc. This mixture form gave a quicker rise (after two injections) in cross-reactive (with Hc) Ab titer as compared to other peptide mixtures or the individual peptides, and sustained in BALB/c a high Ab titer upon further booster injections. Some of the regions that elicited crossreactive immunity to Hc have sequence similarity to other clostridial toxins, suggesting that one or more of these synthetic peptides might provide cross-protection against those toxins.

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Citations

Feb 20, 2002·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·K R Aoki, B Guyer
Sep 10, 2009·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Ki Seo KimSeong Taek Kim
Nov 3, 2009·Biologicals : Journal of the International Association of Biological Standardization·Abdoulreza Agheli MansourNima Farhadi
Jun 19, 2015·Toxicon : Official Journal of the International Society on Toxinology·M Z Atassi
Oct 23, 2008·Movement Disorders : Official Journal of the Movement Disorder Society·Joerg Mueller
Aug 28, 2012·Toxicon : Official Journal of the International Society on Toxinology·Alexey ZdanovskyMaria Zdanovskaia
Feb 7, 2008·Expert Review of Vaccines·Laura M ZarebskiAlessandro Sette
Mar 18, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·M Zouhair Atassi
Nov 28, 2007·Plastic and Reconstructive Surgery·Ki Young Ahn, Seong Taek Kim
Feb 7, 2007·Expert Review of Vaccines·Kohtaro FujihashiDavid W Pascual
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryoki KobayashiKohtaro Fujihashi
Sep 28, 2007·Molecular Immunology·Behzod Z DolimbekM Zouhair Atassi

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