Antibodies from a DNA peptide vaccination decrease the brain amyloid burden in a mouse model of Alzheimer's disease

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Jan G SchiltzKarin Moelling

Abstract

The neuropathology of Alzheimer's disease(AD) is characterized by the accumulation of amyloid peptide Abeta in the brain derived from proteolytic cleavage of the amyloid precursor protein (APP). Vaccination of mice with plasmid DNA coding for the human Abeta42 peptide together with low doses of preaggregated peptide induced antibodies with detectable titers after only 2 weeks. One serum was directed against the four aminoterminal amino acids DAEF and differs from previously described ones. Both immune sera and monoclonal antibodies solubilized preformed aggregates of Abeta42 in vitro and recognized amyloid plaques in brain sections of mice transgenic for human APP. Passive immunization of transgenic AD mice caused a significant and rapid reduction in brain amyloid plaques within 24 h. The combined DNA peptide vaccine may prove useful for active immunization with few inoculations and low peptide dose which may prevent the recently described inflammatory reactions inpatients. The monoclonal antibodies are applicable for passive immunization studies and may lead to a therapy of AD.

References

Apr 6, 1995·Nature·A M KriegD M Klinman
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·B SolomonT Katzav
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·B SolomonE Hanan-Aharon
Feb 27, 1999·Human Gene Therapy·J SchultzK Moelling
Mar 31, 2000·Immunology Today·I A Ramshaw, A J Ramsay
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·D FrenkelB Solomon
Mar 17, 2001·Intervirology·J SchultzK Mölling
Mar 17, 2001·Intervirology·J I Sin, D B Weiner
Mar 29, 2001·Physiological Reviews·D J Selkoe
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·R B DeMattosD M Holtzman
Jan 11, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R WeberK Moelling
Jan 12, 2002·Nature Medicine·Alan Dove
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian O'Nuallain, Ronald Wetzel
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Claude NicolauRobert J Hopkins
Oct 3, 2002·Nature Reviews. Neuroscience·Dale Schenk
Oct 26, 2002·Science·Dennis J Selkoe
Nov 16, 2002·Science·M PfeiferM Jucker
Mar 27, 2003·Trends in Molecular Medicine·Jean-Cosme DodartSteven M Paul
Oct 23, 2003·Seminars in Oncology·Jedd D WolchokHoward I Scher
Nov 1, 2003·Science·Alon Monsonego, Howard L Weiner

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Citations

Feb 16, 2010·Aging Clinical and Experimental Research·Francesco PanzaAlberto Pilotto
Dec 17, 2009·Brain Structure & Function·Thomas Wisniewski, Allal Boutajangout
Apr 28, 2006·Nature Reviews. Immunology·Howard L Weiner, Dan Frenkel
Apr 30, 2008·Rejuvenation Research·Yoshio Okura, Yoh Matsumoto
Jun 15, 2005·Expert Opinion on Biological Therapy·Mary E BrownJoanne McLaurin
Mar 25, 2006·Journal of the Neurological Sciences·Baoxi QuRoger N Rosenberg
May 18, 2010·Biochimica Et Biophysica Acta·Thomas Wisniewski, Einar M Sigurdsson
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Jun 19, 2007·Journal of the Neurological Sciences·Bao-Xi QuRoger N Rosenberg
Nov 14, 2007·Expert Review of Neurotherapeutics·Cheryl A Hawkes, Joanne McLaurin
Feb 23, 2007·Drugs & Aging·Adele WoodhouseJames C Vickers
Nov 14, 2007·Expert Review of Neurotherapeutics·Rawan Tarawneh, James E Galvin

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