PMID: 7516416Jul 1, 1994Paper

Antibodies inhibit the protease-mediated processing of a malaria merozoite surface protein

The Journal of Experimental Medicine
Michael J BlackmanA A Holder

Abstract

When merozoites of the malaria parasite Plasmodium falciparum are released from infected erythrocytes and invade new red cells, a component of a protein complex derived from the merozoite surface protein 1 (MSP-1) precursor undergoes a single proteolytic cleavage known as secondary processing. This releases the complex from the parasite surface, except for a small membrane-bound fragment consisting of two epidermal growth factor (EGF)-like domains, which is the only part of MSP-1 to be carried into invaded erythrocytes. We report that, a group of monoclonal antibodies specific for epitopes within the EGF-like domains, some interfere with secondary processing whereas others do not. Those that most effectively inhibit processing have previously been shown to prevent invasion. Other antibodies, some of which can block this inhibition, not only do not prevent invasion but are carried into the host cell bound to the merozoite surface. These observations unequivocally demonstrate that the binding of antibody to the COOH-terminal region of MSP-1 on the merozoite surface may not be sufficient to prevent erythrocyte invasion, and show that the interaction of different antibodies with adjacent epitopes within the EGF-like domains of MSP-...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M LewM Sheppard
Dec 1, 1987·Experimental Parasitology·G S Hui, W A Siddiqui
Nov 1, 1993·Molecular and Biochemical Parasitology·M J BlackmanA A Holder
May 1, 1993·Molecular and Biochemical Parasitology·L H MillerT F McCutchan

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Citations

May 1, 1996·Parasitology Today·A A Holder, E M Riley
May 10, 2000·Molecular and Biochemical Parasitology·S SondaA Hemphill
May 30, 2001·Molecular and Biochemical Parasitology·C G BlackR L Coppel
Sep 12, 2001·Molecular and Biochemical Parasitology·T M DalyL W Bergman
May 3, 2002·Molecular and Biochemical Parasitology·Isabelle DelrieuAnthony A Holder
Nov 7, 1999·Molecular and Biochemical Parasitology·K Louie, P A Conrad
Feb 28, 2001·Nature Biotechnology
May 7, 2009·Immunology and Cell Biology·Jack S Richards, James G Beeson
Nov 2, 2005·Nature Reviews. Microbiology·Richard J Pleass, Anthony A Holder
Oct 26, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Kumar
Sep 13, 2002·Immunology and Cell Biology·Jiraprapa WipasaMichael F Good
Feb 27, 2001·Current Opinion in Hematology·C E Chitnis

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