PMID: 36615May 1, 1979

Antibodies raised against purified beta-adrenergic receptors specifically bind beta-adrenergic ligands

Proceedings of the National Academy of Sciences of the United States of America
M G CaronR J Lefkowitz

Abstract

Antibodies raised against purified beta-adrenergic receptors themselves specifically bind beta-adrenergic ligands. Digitonin-solubilized frog (Rana pipiens) erythrocyte beta-adrenergic receptors, purified 100- to 200-fold by adsorption to an alprenolol-agarose affinity support and specifically eluted from the affinity resin by 1-100 mM (+/-)-isoproterenol, were used to immunize six rabbits. All immune sera, in contrast to preimmune sera, bound the beta-adrenergic antagonist [(3)H]Dihydroalprenolol binding activity was due to immunoglobulins. By competition studies, antibody [(3)H]dihydroalprenolol binding was found to display a specificity and stereoselectivity resembling that of the beta-adrenergic receptor, [i.e., (-)-isoproterenol > (-)-epinephrine > (-)-norepinephrine; alprenolol approximately propranolol > phentolamine = aloperidol; and (-) isomers of both agonists and antagonists 10-100 times more potent than (+) isomers]. A portion of the [(3)H]dihydroalprenolol binding antibodies could be specifically adsorbed onto purified frog erythrocyte membranes, whereas Xenopus and human erythrocyte membranes, both of which are almost devoid of beta-adrenergic receptors, were ineffective in adsorbing [(3)H]dihydroalprenolol bindin...Continue Reading

References

Dec 29, 2000·Progress in Neuro-psychopharmacology & Biological Psychiatry·S MaltaisP Falardeau
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A B SchreiberA D Strosberg
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J PithaM G Caron
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K P CampbellS D Kahl
Apr 30, 1981·Biochemical and Biophysical Research Communications·P O CouraudA D Strosberg

Citations

Jan 1, 1978·Biochemical Pharmacology·J HoebekeA D Strosberg
Apr 16, 1976·Science·R P Shiu, H G Friesen
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K Sege, P A Peterson
Jun 16, 1978·Science·S JacobsP Cuatrecasas
Mar 1, 1975·Clinical Endocrinology·R HallE D Mukhtar
Mar 1, 1976·Endocrinology·L J Grota, G M Brown
May 25, 1973·Science·J Patrick, J Lindstrom

Related Concepts

Rana pipiens
Binding Sites, Antibody
Salientia
Digitonin
Immune Sera
Adrenergic beta-Agonists
Adsorption
Phosphoglycerate Dehydrogenase Activity
Immunoglobulins
ADRB2 gene

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