Antibodies reactive to cleaved sites in complement proteins enable highly specific measurement of soluble markers of complement activation

Molecular Immunology
Anna M BlomMarcin Okroj

Abstract

An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies reactive to C4d and C5b fragments enabled us to set up highly specific sandwich ELISAs, which ensured trustful measurements without false positive readouts characteristic for some of the widely used assays.

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Citations

Jun 27, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Elena B VolokhinaTom Eirik Mollnes
Sep 13, 2016·Journal of Immunoassay & Immunochemistry·Kheir Eddine KerbouaDjamila Batouche
Jun 24, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leonie M VogtAnna M Blom
Aug 11, 2017·Nature Reviews. Rheumatology·Leendert A TrouwAnna M Blom
Jun 4, 2016·Current Opinion in Infectious Diseases·Esther Benamu, José G Montoya
Jun 28, 2019·Lupus Science & Medicine·Tineke KraaijYk Onno Teng
Sep 16, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Camilla Melin FürstAnna M Blom
Nov 20, 2016·Arthritis Research & Therapy·Marcin OkrójRoger Hesselstrand
Feb 22, 2018·Oncotarget·Daniel AjonaRuben Pio
Dec 7, 2017·Arthritis Research & Therapy·Myriam MartinAnna M Blom
Mar 13, 2021·Journal of Medical Virology·Qing YeDezhao Lu

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