PMID: 7517679Jun 1, 1994

Antibodies specific for the neuronal form of the Src protein elicited by an antigenized antibody

DNA and Cell Biology
P RigaudyW Eckhart

Abstract

To elicit antibodies directed specifically against the neuron-specific form of the c-src gene product, pp60c-src(+), we used an antigenized antibody comprising a decamer containing the amino acid sequence specific to pp60c-src(+) inserted into the third hypervariable loop of the heavy (H)-chain variable (V)-region. This was used to raise anti-idiotype antibodies reacting with the peptide epitope in rabbits. The antisera reacted with pp60c-src(+), as judged by immune blotting, immunoprecipitation, immune complex kinase assay, and indirect immunofluorescence staining, but did not react with the fibroblast form of the c-src gene product, pp60c-src. Antigenized antibody is a useful approach for producing antibodies able to distinguish between isoforms of the same gene product and specific for the neuronal form of the Src protein.

References

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Citations

Dec 1, 1992·Immunological Reviews·M ZanettiR Billetta
Mar 14, 2002·International Reviews of Immunology·M Gerloni, M Zanetti
Apr 3, 2007·The Journal of Biological Chemistry·Franco OnofriFabio Benfenati

Related Concepts

Anti-Antibodies
Antibody Specificity
Antigenic Specificity
DNA, Double-Stranded
Neurons
Proteins, Recombinant DNA
Proto-Oncogene Protein pp60 (c-src)
3T3 Cells

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