Antibodies to a CA 19-9 Related Antigen Complex Identify SOX9 Expressing Progenitor Cells In Human Foetal Pancreas and Pancreatic Adenocarcinoma

Scientific Reports
Alison M FarleyMartin F Pera

Abstract

The Sialyl Lewis A antigen, or CA 19-9, is the prototype serum biomarker for adenocarcinoma of the pancreas. Despite extensive clinical study of CA 19-9 in gastrointestinal malignancies, surprisingly little is known concerning the specific cell types that express this marker during development, tissue regeneration and neoplasia. SOX9 is a transcription factor that plays a key role in these processes in foregut tissues. We report the biochemistry and tissue expression of the GCTM-5 antigen, a pancreatic cancer marker related to, but distinct from, CA19-9. This antigen, defined by two monoclonal antibodies recognising separate epitopes on a large glycoconjugate protein complex, is co-expressed with SOX9 by foregut ductal progenitors in the developing human liver and pancreas, and in pancreatic adenocarcinoma. These progenitors are distinct from cell populations identified by DCLK1, LGR5, or canonical markers of liver and pancreatic progenitor cells. Co-expression of this antigen complex and SOX9 also characterises the ductal metaplasia of submucosal glands that occurs during the development of Barrett's oesophagus. The GCTM-5 antigen complex can be detected in the sera of patients with pancreatic adenocarcinoma. The GCTM-5 epitop...Continue Reading

References

Jun 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M MiyagiwaH Sasaki
Jun 23, 2004·Biochemical and Biophysical Research Communications·Valéry GmyrJulie Kerr-Conte
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Philip A SeymourMaike Sander
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Randall E BrandAnna E Lokshin
Sep 29, 2011·BMC Developmental Biology·Peter S VestentoftHanne C Bisgaard
Oct 11, 2011·The International Journal of Biochemistry & Cell Biology·Erin McDonaldRennian Wang
Dec 3, 2011·The Journal of Clinical Investigation·Maximilian Reichert, Anil K Rustgi
Mar 1, 2012·Nature Reviews. Gastroenterology & Hepatology·Vincenzo CardinaleDomenico Alvaro
Aug 10, 2012·Development·Philip A SeymourMaike Sander
May 1, 2013·Diabetes·Rachel E JenningsNeil A Hanley
May 3, 2013·The Journal of Clinical Investigation·Yoshiya Kawaguchi
Sep 4, 2013·Gastroenterology·Kathleen E DelgiornoHoward C Crawford
Apr 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Branden D TarlowMarkus Grompe
May 28, 2014·Virchows Archiv : an International Journal of Pathology·David R BraxtonAlton B Farris
Jun 19, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Darragh P O'BrienJohn F Timms
Oct 18, 2014·Islets·Rachel J SalisburyNeil A Hanley
Nov 5, 2014·Nature Reviews. Gastroenterology & Hepatology·Stuart A C McDonaldMarnix Jansen
Jul 21, 2015·Nature Cell Biology·Wei-Yu LuStuart J Forbes
Nov 5, 2015·Advances in Experimental Medicine and Biology·Salvatore ScaràRoberto Scatena
Nov 27, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Julia Köhn-GaoneJanina Elke Eleonore Tirnitz-Parker
Nov 16, 2016·The American Journal of Gastroenterology·Sukhwinder KaurSurinder K Batra
Feb 22, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yuichi HirataMasaru Yoshida
Mar 9, 2017·Nature Reviews. Gastroenterology & Hepatology·Peter Storz
Apr 5, 2017·Journal of the National Cancer Institute·Michela CapelloSamir M Hanash
Jul 14, 2017·Science Translational Medicine·Jungsun KimKenneth S Zaret

❮ Previous
Next ❯

Citations

May 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mirza Muhammad Fahd QadirJuan Domínguez-Bendala

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM098791
GM103694

Methods Mentioned

BETA
affinity purification
pulldowns
RNA-seq
biopsies
biopsy
ELISA
immunoprecipitation
Assay
fluorescence activated cell sorter
pull downs

Software Mentioned

Mascot

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.