Antibodies to Conserved Surface Polysaccharides Protect Mice Against Bacterial Conjunctivitis

Investigative Ophthalmology & Visual Science
Tanweer ZaidiGerald B Pier

Abstract

Bacterial conjunctivitis is a major problem in ocular health. Little is known about protective immune effectors in the conjunctiva. We evaluated whether opsonic antibody to the conserved surface/capsular polysaccharide poly-N-acetyl glucosamine (PNAG) expressed by Streptococcus pneumoniae and Staphylococcus aureus was protective against bacterial conjunctivitis, as well as an antibody to the Pseudomonas aeruginosa surface polysaccharide alginate. Bacteria were injected directly into the conjunctivae of either A/J mice or into conjunctivae of wild type C57Bl/6 mice for comparisons to responses of recombination activating gene 1-knock out (RAG 1 KO) or germ-free mice in the C57Bl/6 genetic background. Human IgG1 monoclonal antibodies (MAb) to either PNAG or alginate were administered as follows: direct injection of 10 μg into the conjunctivae or topical application onto the cornea 4, 24, and 32 hours post infection; or intraperitoneal injection of 200 μg 18 hours prior to and then 4, 24, and 32-hours postinfection. After 48 hours, eyes were scored for pathology, mice were euthanized, and CFU/conjunctiva was determined. All methods of antibody administration reduced S. pneumoniae, S. aureus, or P. aeruginosa pathology and bacteria...Continue Reading

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Citations

Apr 6, 2019·Current Opinion in Infectious Diseases·Michael P MotleyBettina C Fries
Nov 27, 2020·Computational and Structural Biotechnology Journal·Hoai T T NguyenMichael Otto
Nov 11, 2019·Microorganisms·Erin T LivingstonMichelle C Callegan
Apr 22, 2021·The Biochemical Journal·J Sebastian TemmeJeffrey C Gildersleeve

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Methods Mentioned

BETA
transgenic
ELISA

Clinical Trials Mentioned

NCT03222401
NCT02853617
NCT02486770
NCT03027609

Software Mentioned

Prism
GraphPad

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