Antibodies to meningococcal H.8 (Lip) antigen fail to show bactericidal activity

Canadian Journal of Microbiology
A K BhattacharjeeW D Zollinger

Abstract

Purified H.8 (Lip) antigen was coupled to tresyl-activated Sepharose 4B and used in affinity columns to purify anti-Lip antibodies from convalescent patient sera and from immune rabbit sera. Affinity-purified anti-Lip antibodies isolated from two convalescent patient sera contained 1000 and 1280 ELISA units of antibody and included antibodies of IgG, IgA, and IgM isotypes. An anti-Lip mouse monoclonal ascites (2-1-CA2) had 28,400 ELISA units of antibody. Bactericidal assays were performed using three different case strains of Neisseria meningitidis group B, namely 44/76, 8532, and 8047. Neither preparation of purified human anti-Lip antibodies had detectable bactericidal activity against strains 44/76 and 8532, but one of the two had a titer of 1:4 against strain 8047. Anti-Lip antibodies that were purified from immune rabbit serum and contained 1600 ELISA units of anti-Lip antibodies also failed to show detectable bactericidal activity. The rabbits were immunized with purified Lip antigen and showed specific antibody levels of 2000-2200 units by ELISA, but even the unfractionated sera had little or no bactericidal activity against the test strains. The high titer mouse monoclonal ascites had no bactericidal activity against th...Continue Reading

Citations

Jan 21, 2000·FEMS Immunology and Medical Microbiology·G TroncosoC M Ferreirós
Jan 1, 1993·The Journal of Applied Bacteriology·A Robinson, J Melling
Jan 19, 2006·Clinical Microbiology Reviews·Lee H Harrison
Nov 13, 2003·BMC Microbiology·Philip JordanNigel J Saunders
Apr 7, 1997·The Journal of Experimental Medicine·D MartinB R Brodeur
May 25, 1999·Infection and Immunity·M PlanteD Martin
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