Antibodies to the Muscarinic Acetylcholine Receptor M3 in Primary Biliary Cholangitis Inhibit Receptor Function on Cholangiocytes.

Frontiers in Immunology
Christian MayerR Klein

Abstract

Background and Aims: In primary biliary cholangitis (PBC), antibodies to a peptide of the muscarinic acetylcholine receptor 3 (mAChR3) have been described. Since the mAChR3 is expressed on cholangiocytes and mAChR3-signaling is involved in the pathogenesis of chronic inflammatory biliary diseases, we wanted to investigate whether anti-mAChR3-antibodies influence the function of the receptor and the proliferative response of cholangiocytes. Methods: Immunoglobulins were isolated by ammonium sulfate precipitation using sera from patients with PBC (n = 63) and with other chronic liver disorders (n = 150). All immunoglobulins were analyzed by a luminometric assay using Chinese hamster ovary (CHO) cells overexpressing the mAChR3 and cholangiocytes (TFK-1-cells) expressing the receptor constitutively. Cell proliferation was measured by 3H-thymidine assay. PBC patients were also analyzed in the follow-up. Results: Antibodies inhibiting the mAChR3 were found in 49 and 79% of PBC patients using CHO-cells or TFK-1-cells, respectively, but only in up to 26% of controls (p < 0.01). Stimulatory antibodies were hardly detected. Antibody reactivity only marginally changed during the course of the disease, independently of the choice of treatm...Continue Reading

References

Jun 1, 1988·Journal of Hepatology·A KyriatsoulisK H Meyer zum Büschenfelde
Sep 1, 1996·The American Journal of Physiology·E L WatsonF J Dowd
Sep 18, 1997·The Journal of Clinical Investigation·D AlvaroA Benedetti
Mar 18, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·C Pérez LeirósE S Borda
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·V BaubetP Brúlet
Feb 1, 2002·Transplantation·Jill TinmouthLeslie Lilly
Oct 3, 2002·Gastroenterology·Fiona GoldblattSally A Waterman
Aug 28, 2004·Clinical & Developmental Immunology·Gordon D BensonM Eric Gershwin
Sep 28, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jingchao LiKyungpyo Park
Jan 13, 2005·Journal of Hepatology·Domenico AlvaroEugenio Gaudio
Sep 7, 2005·Seminars in Liver Disease·Giammarco FavaGianfranco Alpini
Oct 4, 2005·Arthritis and Rheumatism·Luke DawsonTom Gordon
May 6, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·O BachmannU Seidler
Jun 23, 2006·The New England Journal of Medicine·Silvia Svegliati BaroniArmando Gabrielli
May 30, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·László KovácsViktória Boda
Jun 6, 2008·World Journal of Gastroenterology : WJG·Dimitrios-P BogdanosDiego Vergani
Jun 13, 2008·Clinical Reviews in Allergy & Immunology·Marco MarzioniAntonio Benedetti
Jul 2, 2009·Current Opinion in Rheumatology·Nikolay P Nikolov, Gabor G Illei
Sep 26, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jagmohan SinghSatish Rattan
Feb 25, 2010·World Journal of Gastroenterology : WJG·Sandra BraunReinhild Klein
Feb 26, 2010·The New England Journal of Medicine·Rebecca S Bahn
Apr 24, 2010·Seminars in Thrombosis and Hemostasis·Roland JahnsMartin J Lohse
Aug 20, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ulrich BeuersRonald P J Oude Elferink
Sep 21, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Simon HohenesterUlrich Beuers
Feb 4, 2012·Current Medicinal Chemistry·B TrzaskowskiS Filipek
Apr 23, 2014·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Hiroto TsuboiTakayuki Sumida
Jun 22, 2017·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Franziska DurchscheinPeter Fickert
Oct 23, 2018·Best Practice & Research. Clinical Gastroenterology·Annarosa Floreani, Nora Cazzagon

❮ Previous
Next ❯

Citations

Oct 6, 2021·European Journal of Immunology·Larissa Nogueira de AlmeidaRudolf Armin Manz

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assays
electrophoresis
transfection
enzyme-linked immunosorbent assay
ELISA
biopsies

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.