Antibodies to triosephosphate isomerase in patients with neuropsychiatric lupus

Biochemical and Biophysical Research Communications
Hiroshi WatanabeYukio Sato

Abstract

Sera from patients with neuropsychiatric lupus (NP lupus) were screened for antibodies to mouse choroid plexus cell line ECPC-4 by Western blotting. A 29-kDa protein band detected in NP lupus sera was identified as triosephosphate isomerase (TPI). Using Western blotting with TPI, TPI was confirmed as the reactive molecule in sera (6 of 14 samples) and in cerebrospinal fluids (1 of 2 samples) of patients with NP lupus. Enzyme-linked immunosorbent assay with TPI showed that the serum anti-TPI antibody index of 89.8 (SD, 70.1) in NP lupus group was significantly higher than in systemic lupus erythematosus without NP manifestations, 34.6 (29.6); scleroderma, 38.2 (39.9); polymyositis/dermatomyositis, 42.1 (51.5); and control, 31.7 (27.4) groups (p<0.02). Sensitivity, specificity, and positive and negative likelihood ratios of serum anti-TPI antibody index for NP lupus were 42.9%, 94.7%, 8.1%, and 0.6%, respectively. These results suggest that anti-TPI antibodies are closely associated with NP lupus.

References

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Citations

Feb 2, 2013·Applied Microbiology and Biotechnology·Jian WangShuo Wang
Jan 30, 2007·Seminars in Arthritis and Rheumatism·Gisele Zandman-GoddardYehuda Shoenfeld
May 4, 2018·BioMed Research International·Zeineb ZianMohcine Bennani Mechita
Oct 30, 2018·Current Protein & Peptide Science·Mónica Rodríguez-Bolaños, Ruy Perez-Montfort

Related Concepts

Autoantibodies
Autoantigens
Diagnosis, Psychiatric
Lupus Erythematosus, Systemic
Nervous System Disorder
Triose-Phosphate Isomerase

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