Antibody against single-stranded DNA useful for detecting apoptotic cells recognizes hexadeoxynucleotides with various base sequences

Journal of Biochemistry
Y KawaradaT Sugiyama

Abstract

Our previous study demonstrated that an antibody against single-stranded DNA could detect apoptotic cells [Naruse et al. (1994) Histochemistry 101, 73-78]. In this paper we describe the development of an improved method for the production of the antibody and investigations into the antigenic determinants of the antibody so that it could be of practical use for detecting apoptotic cells. Rabbits, hyperimmunized with complexes of alkaline-denatured calf thymus DNA and methylated bovine serum albumin, produced an IgG antibody to single-stranded DNA. Analysis by sandwich ELISA using various naturally occurring nucleic acids revealed that the antibody was specific to single-stranded DNA. Furthermore, using synthetic polymers in the assay, it was found that the antibody could recognize single-stranded DNA with various base sequences. Gel electrophoresis retardation assays, with synthetic oligodeoxynucleotides with differing lengths of single-stranded DNA, indicated that a hexadeoxynucleotide constituted the minimum size of the antigenic determinants, and suggested that the antibody probably consists of several antibodies which recognize hexadeoxynucleotides with various base sequences. Western blot analysis demonstrated that the anti...Continue Reading

Citations

Sep 15, 2000·Journal of Immunological Methods·I VermesC Reutelingsperger
Mar 31, 2007·Development, Growth & Differentiation·Machiko IidaTakashi Matsuzaki
Nov 13, 2012·The Journal of Clinical Investigation·Zijing ShengYusaku Nakabeppu
May 23, 2008·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·T Schneider, H Dienemann
Mar 18, 2008·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·M StefflW M Amselgruber
May 4, 2007·The EMBO Journal·Pierre-Olivier FrappartPeter J McKinnon
Dec 19, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Yoshitomo ChiharaHiroki Kuniyasu
Apr 7, 2007·Journal of Neurosurgery·Nobutake SadamasaNobuo Hashimoto
Feb 19, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Tomoo ItohKazuo Nagashima
Oct 27, 2006·International Journal of Cancer. Journal International Du Cancer·Kaeko HiraiHiroki Kuniyasu
Nov 15, 2003·Stroke; a Journal of Cerebral Circulation·Nobutake SadamasaNobuo Hashimoto
Apr 1, 2000·Journal of Neuropathology and Experimental Neurology·M MizuguchiO Hino

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis