Antibody-Antigen Interaction Dynamics Revealed by Analysis of Single-Molecule Equilibrium Fluctuations on Individual Plasmonic Nanoparticle Biosensors

ACS Nano
Srdjan S AćimovićTomasz J Antosiewicz

Abstract

Antibody-antigen interactions are complex events central to immune response, in vivo and in vitro diagnostics, and development of therapeutic substances. We developed an ultrastable single-molecule localized surface plasmon resonance (LSPR) sensing platform optimized for studying antibody-antigen interaction kinetics over very long time scales. The setup allowed us to perform equilibrium fluctuations analysis of the PEG/anti-PEG interaction. By time and frequency domain analysis, we demonstrate that reversible adsorption of monovalently bound anti-PEG antibodies is the dominant factor affecting the LSPR fluctuations. The results suggest that equilibrium fluctuation analysis can be an alternative to established methods for determination of interaction rates. In particular, the methodology is suited to analyze molecular systems whose properties change during the initial interaction phases, for example, due to mass transport limitations or, as demonstrated here, because the effective association rate constant varies with surface concentration of adsorbed molecules.

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Citations

May 10, 2019·Science and Technology of Advanced Materials·Kouichi Shiraishi, Masayuki Yokoyama
Jun 21, 2020·Nature Communications·Ping ZhangChunhai Fan
Feb 24, 2021·Analytical Chemistry·Peng Fei GaoCheng Zhi Huang
May 1, 2021·Biosensors·Elba Mauriz, Laura M Lechuga
Mar 25, 2021·Annual Review of Analytical Chemistry·Andreas Dahlin
Sep 7, 2021·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Teresa StaniszewskaSeweryn Morawiec

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