Antibody-Based Assays for Phenotyping of Extracellular Vesicles

BioMed Research International
Lotte Hatting PugholmMalene Jørgensen

Abstract

Extracellular vesicles (EVs) are a heterogeneous population of membrane-enclosed vesicles. EVs are recognized as important players in cell-to-cell communication and are described to be involved in numerous biological and pathological processes. The fact that EVs are involved in the development and progression of several diseases has formed the basis for the use of EV analysis in a clinical setting. As the interest in EVs has increased immensely, multiple techniques have been developed aiming at characterizing these vesicles. These techniques characterize different features of EVs, like the size distribution, enumeration, protein composition, and the intravesicular cargo (e.g., RNA). This review focuses on techniques that exploit the specificity and sensitivity associated with antibody-based assays to characterize the protein phenotype of EVs. The protein phenotype of EVs can provide information on the functionality of the vesicles and may be used for identification of disease-related biomarkers. Thus, protein profiling of EVs holds great diagnostic and prognostic potential.

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Citations

Jul 26, 2017·Cell Proliferation·Zi-Chen HaoShuo-Gui Xu
Dec 9, 2017·Acta Pharmacologica Sinica·Saheli SamantaJohnson Rajasingh
Mar 8, 2018·Journal of Extracellular Vesicles·Sabrina RoyPamela S Jones
May 23, 2017·Bioengineering & Translational Medicine·Sahil InamdarKaushal Rege
Jul 1, 2017·Thrombosis and Haemostasis·Victoria C RidgerPaul Harrison
Jan 13, 2021·International Journal of Molecular Sciences·Edna SoaresFrederico C Pereira

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
surface plasmon resonance
chip
Assay
NMR
membrane filtration
ELISA

Software Mentioned

nPLEX

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