Antibody-conjugated ferromagnetic nanoparticles with lateral flow test strip assay for rapid detection of Campylobacter jejuni in poultry samples

International Journal of Food Microbiology
Wanwisa PoonlapdechaPravate Tuitemwong

Abstract

The aim of this study was to develop a nanoparticle-based cell capture system combined with a lateral flow test strip (LFT) assay for rapid detection of Campylobacter jejuni from poultry samples. The developed assay was bench-marked against the standard modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) method according to ISO16140:2003 procedures. The synthesized ferromagnetic nanoparticles (FMNs) were modified with glutaraldehyde, then functionalized with polyclonal antibodies for specific C. jejuni capture and concentration from poultry samples. After lysing captured cells, DNA from C. jejuni was amplified by PCR using the primers designed to target the hipO gene, and the PCR amplicons were detected with the lateral flow test strip assay. Following the ISO16140:2003 guidelines, the relative detection limit, and the inclusivity and exclusivity tests were determined. The results showed that the limit of detection (LOD) of the assay was 100 or 1 cfu/ml with C. jejuni in pure culture and 101-102 cfu/ml with target cells spiked in poultry sample. In addition, the inclusivity and exclusivity tests were found to be 100%. Using field chicken samples (n = 60), the assay showed relative accuracy, relative specificity, and relati...Continue Reading

Citations

May 14, 2020·Diagnostics·Amanda MoyanoM Carmen Blanco-López
Feb 8, 2019·3 Biotech·Jaspreet SinghShivesh Sharma
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Carolina Ripolles-AvilaJosé-Juan Rodríguez-Jerez
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zidane QriouetZineb Qmichou

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