Antibody-dependent cell-mediated cytotoxic activity in syngeneic moouse ascites tumors.

International Journal of Cancer. Journal International Du Cancer
D E TraceyI P Witz

Abstract

Eight different syngeneic murine ascites tumor preparations were tested for their ability to lyse antibody-coated chicken erythrocytes in an assay for antibody-dependent cell-mediated cytotoxicity (ADCC). All of the tumor preparations demonstrated significant ADCC effector activity, even at effector-cell:target-cell ratios of less than 1:1. In contrast, none of eight in vitro murine tumor-cell lines showed any consistent ability to function as ADCC effector cells. Several cell fractionation experiments were performed to ascertain the nature of the effector cells in the ascites tumors. Adherent cells were removed from tumor preparations by passage of cells over columns of Degalan plastic beads and 19S Fc-receptor-bearing cells were removed by sedimentation of EA rosette-forming cells. Although each of these procedures depleted only a minority of the total cells, all the ADCC activity was simultaneously removed in each case. Depletion of phagocytic cells by treatment of tumor preparations with carbonyl iron and magnetism only slightly diminished the ADCC activity. These results indicate that the ADCC activity associated with ascites tumors is due to effector cells most probably of host origin, but possibly representing an atypica...Continue Reading

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Citations

Jan 1, 1992·Immunologic Research·I P Witz, M Ran
Jun 15, 1975·International Journal of Cancer. Journal International Du Cancer·R S KerbelE V Elliott
Oct 15, 1976·International Journal of Cancer. Journal International Du Cancer·R S Kerbel, H F Pross
Aug 1, 1976·European Journal of Immunology·G R BraslawskyI P Witz
Jan 15, 1980·International Journal of Cancer. Journal International Du Cancer·V SanterP K Lala

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