Antibody-dependent, cell-mediated cytotoxicity against human red blood cells: correlation of effector cell type with enzymatic alteration of the target cell surface

European Journal of Immunology
I YustN Goldsher

Abstract

Target cell factors, which contribute to the determination of the effector cell type in an antibody-dependent, cell-mediated cytotoxicity system were studied. Human red blood cells (RBC) were treated with papain and investigated by transmission and scanning electron microscopy (TEM and SEM). Both untreated (native) and treated 0 Rh+ RBC were labeled with 51Cr, sensitized with anti-D immunoglobulin and incubated with unfractionated autologous peripheral blood mononuclear cells. With native RBC, immune lysis was proportional to the number of phagocytes: enrichment of effectors in phagocytes increased lysis, while depletion of phagocytes from effectors decreased lysis. Following papain treatment of target RBC, lysis by unfractionated mononuclear effectors was markedly augmented; since this effect was not diminished by decrease of phagocytes to less than 2%, the augmented lysis was not due to the number of phagocytic effectors. TEM and SEM of enzyme-treated RBC showed spherocytosis with varying degrees of crenation and blurring and irregularities of the cellular membranes. The results suggest that papain-induced alterations in the target RBC rendered them susceptible to lysis by interaction between anti-D antibody and peripheral bl...Continue Reading

Citations

Oct 29, 1976·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C J Sanderson, J A Thomas
Sep 1, 1975·Scandinavian Journal of Immunology·H R MacDonaldS A Zawodnik
Dec 1, 1978·British Journal of Haematology·B Frisch, S M Lewis
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M M Kay
Jan 1, 1974·Advances in Immunology·J C Cerottini, K T Brunner
Jan 1, 1970·International Archives of Allergy and Applied Immunology·W Pollack, R P Reckel
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen

Related Concepts

Pathologic Cytolysis
Tissue Membrane
Immunoglobulin Activity
Peripheral Blood
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Antigen D, Rh Blood Group
Peripheral Blood Mononuclear Cells (Lab Test)
Red blood cells, blood product
Blurred Vision
Immune Sera

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Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.