PMID: 9420226Jan 7, 1998Paper

Antibody-dependent cellular cytotoxicity directed against cells expressing human immunodeficiency virus type 1 envelope of primary or laboratory-adapted strains by human and chimpanzee monoclonal antibodies of different epitope specificities

Journal of Virology
O Alsmadi, S A Tilley

Abstract

The characteristics of antibody-dependent cellular cytotoxicity (ADCC) directed by a panel of human and chimpanzee antienvelope (anti-Env) monoclonal antibodies (MAbs) of different epitope specificities were studied; this was accomplished by using target cells expressing human immunodeficiency virus type 1 (HIV-1) Envs of either primary or laboratory-adapted strains. Human MAbs of similar apparent affinities (1 x 10(9) to 2 x 10(9) liters/mol) against either a "cluster II"-overlapping epitope of gp41 or against the CD4 binding site, V3 loop, or C5 domain of gp120 directed substantial and comparable levels of specific lysis against targets infected with laboratory-adapted strains of HIV-1. As expected, those MAbs specific for relatively conserved regions of Env generally exhibited ADCC activity against a broader range of HIV-1 strains than those directed against variable epitopes. Significant ADCC activities of selected MAbs against primary isolate Env-expressing cells were demonstrated. In addition, a new ADCC epitope in the V2 domain of gp120 was defined. CD56+ cells were demonstrated to be the effector cells in these studies by fluorescence-activated cell sorting followed by ADCC assays. Notably, all anti-Env MAbs tested in t...Continue Reading

References

Dec 1, 1989·AIDS Research and Human Retroviruses·D S TylerK J Weinhold
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H K LyerlyK J Weinhold

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Citations

May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Donald N ForthalTran Phan
Jan 27, 2017·Journal of Virology·Timothée BruelOlivier Schwartz
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takeshi YamadaAikichi Iwamoto
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Victor Raul Gómez-RománGoar Gevorkian
Feb 20, 2019·PLoS Pathogens·Katherine L WilliamsJulie Overbaugh

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