PMID: 8592685Nov 1, 1995Paper

Antibody dependent, complement mediated liver uptake of liposomes containing GM1

Pharmaceutical Research
D LiuF Liu

Abstract

We have previously reported that GM1 exhibits an opposite effect on regulating liposome circulation time in mice and rats (Liu et al. Pharm. Res. Vol. 12:508-512 (1995)). Inclusion of GM1 into liposomes significantly prolongs liposome circulation time in mice, while it dramatically decrease the blood half life and increases liver uptake of liposomes in rats. The purpose of this study was to elucidate the mechanism that underlies this phenomenon. Single-pass liver perfusion in vitro and complement mediated liposome lysis assay was used. Serum appeared to play an important role in determining the liver uptake of GM1 liposomes. Specifically, rat serum enhanced the uptake of GM1-containing liposomes by the perfused liver. Such activity was also found in human and bovine serum, but not in mouse serum. Taking human serum as an example, we demonstrated that such serum activity can be blocked by EDTA and EGTA/Mg2+. Antibodies against human IgM and the third component of complement system (C3) also inhibited serum activity. The presence of naturally occurring anti-GM1 antibodies in rats, through the activation of the classic pathway of complement system, is likely the cause of rapid blood clearance of GM1-liposomes. The third component ...Continue Reading

Citations

Mar 28, 2013·Journal of Drug Delivery·Stefano Salmaso, Paolo Caliceti
Oct 1, 2005·Journal of Liposome Research·Xuedong YanJan A A M Kamps
Jun 17, 2006·Journal of Biomedical Materials Research. Part a·A VonarbourgJ P Benoit
Nov 29, 2013·BioMed Research International·Nidhi MishraSumit Arora
Apr 4, 2019·The Journal of Pharmacology and Experimental Therapeutics·S Moein MoghimiA Christy Hunter
Mar 4, 2003·Journal of Cellular and Molecular Medicine·Manuela Voinea, Maya Simionescu
Dec 5, 2013·Pharmaceutics·Okhil K Nag, Vibhudutta Awasthi
Jan 1, 1996·Drug Delivery·T L Whateley
May 12, 2020·Advanced Drug Delivery Reviews·S Moein MoghimiZ Shadi Farhangrazi
Dec 6, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·K OgawaraT Kimura
Jun 6, 2000·Advanced Drug Delivery Reviews·A J Bradley, D V Devine
Jun 6, 2000·Advanced Drug Delivery Reviews·K J WilliamsW V Rodrigueza
Jun 6, 2000·Advanced Drug Delivery Reviews·H KiwadaH Harashima
Jun 6, 2000·Advanced Drug Delivery Reviews·J A Kamps, G L Scherphof
Sep 9, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·K OgawaraT Kimura
Sep 11, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kentaro FurumotoToshikiro Kimura

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