PMID: 2035620May 1, 1991

Antibody-detectable changes in fibrinogen adsorption affecting platelet activation on polymer surfaces

The American Journal of Physiology
E ShibaE W Salzman

Abstract

Reactivity of platelets with an artificial surface exposed to whole blood is correlated with the concentration of adsorbed fibrinogen detectable by antifibrinogen antibodies. To examine the effect on platelets of the organization (distribution, orientation, conformation) of fibrinogen adsorbed on a hydrophobic surface, we studied the binding of polyclonal and monoclonal antifibrinogen antibodies to polyalkyl methacrylate polymers previously exposed to purified fibrinogen solution or diluted plasma and compared the results with platelet retention in methacrylate bead columns. There was an increase in platelet retention following diluted plasma pretreatment, which was eliminated by a polyclonal antibody against fibrinogen or against a gamma-(395-411) peptide from fibrinogen and was reduced by monoclonal antibodies (4A5, 4-2) against other COOH-terminal gamma-chain epitopes. Monoclonal antibody 10E5 against the fibrinogen receptor GpIIb/IIIa totally inhibited platelet retention in the bead columns. Our data suggest that different methacrylate polymers induce different changes in adsorbed fibrinogen, which may interfere with its interaction with platelets, and that platelet retention in a methacrylate bead column involves interacti...Continue Reading

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Related Concepts

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Whole Blood
Fibrinogen
Fibrinogen Assay
Polyclonal antibody
Platelet Membrane Glycoproteins
Methylmethacrylates
Receptors, Fibrinogen
Adsorption

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