Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle

Research in Veterinary Science
Carmen CasalJavier Bezos

Abstract

We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se. The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54-72.00), while the Se of the IFN-γ assays ranged between 60.2% and 92%. The proportion of infected cattle detected by the different antibody detection techniques ranged from 65.5% to 87.6%. Three of the antibody detection techniques yielded a significant higher (p<0.05) Se than that achieved with the official diagnostic techniques. In addition, the interpretation in parallel of cellular and antibody detection techniques reached the highest Se: 98.2% (95% CI, 93.78-99.51) suggesting that the use of diagnostic techniques detecting both cellular and humoral responses could be considered as an alternative in the control of bTB outbreaks in high prevalence sett...Continue Reading

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Citations

Jun 30, 2018·Journal of Fish Diseases·Vladimir LópezJosé de la Fuente
Oct 6, 2017·Clinical and Vaccine Immunology : CVI·Konstantin P LyashchenkoW Ray Waters
Oct 12, 2018·The Journal of Veterinary Medical Science·Ingrid Ieda Fernando de SouzaFlábio Ribeiro Araújo
Jan 19, 2019·Veterinary Medicine and Science·Jose A Infantes-LorenzoFrancisco J Salguero
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Aug 29, 2018·Frontiers in Veterinary Science·Jose A Infantes-LorenzoFrancisco J Salguero
May 31, 2019·Transboundary and Emerging Diseases·Alba Fresco-TaboadaPaloma Rueda
Jul 28, 2020·Frontiers in Veterinary Science·Claudia Arrieta-VillegasBernat Pérez de Val
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Dec 16, 2020·Journal of Immunological Methods·Natalia AlonsoMaría I Romano
Apr 2, 2021·BMC Veterinary Research·Paulo Alex Machado CarneiroJohn B Kaneene
Aug 31, 2021·Frontiers in Veterinary Science·Javier OrtegaMercedes Domínguez
Oct 31, 2021·Transboundary and Emerging Diseases·Jesús Ruiz-CabelloJose L Izquierdo-García

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