PMID: 6982151Jan 1, 1982Paper

Antibody determination by ELISA in rats with retinal S antigen-induced uveoretinitis

Current Eye Research
V Van TuyenB Fortier

Abstract

Enzyme-linked immunosorbent assay was applied to the determination of the serum IgG antibody contents in rats immunized with the organ-specific autoantigen (S antigen) of the retina. Optimal conditions (i.e. S antigen concentration, serum and conjugate dilutions, enzymatic reaction time) were determined. The assay required only a single serum dilution and was well reproducible. It was very sensitive, allowing the detection of low antibody contents in animals immunized with 1 microgram of S antigen. The time course of the antibody response and its variations according to the dose and the species of the immunizing S antigen were considered.

References

Jan 1, 1981·Current Eye Research·Y de KozakJ P Faure

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Citations

Jun 1, 1984·Current Eye Research·J P FaureC Boucheix
Jan 1, 1989·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·F G RobergeR B Nussenblatt
Feb 1, 1997·Chemical Research in Toxicology·C L SevillaL J Marnett
Jul 16, 1985·Biochemical and Biophysical Research Communications·C L SevillaD M Callewaert

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