Antibody-Enabled Antimicrobial Nanocapsules for Selective Elimination of Staphylococcus aureus.

ACS Applied Materials & Interfaces
Kristina IvanovaTzanko Tzanov

Abstract

Targeted bactericide nanosystems hold significant promise to improve the efficacy of existing antimicrobials for treatment of severe bacterial infections, minimizing the side effects and lowering the risk of the development of antibiotic resistance. In this work, we developed antibody-functionalized nanocapsules (NCs) containing antibacterial essential oil (EO) for selective and effective eradication of Staphylococcus aureus. Antibacterial EO NCs were produced via self-assembly nanoencapsulation in the plant-derived protein zein. The obtained EO NCs were decorated with aminocellulose to provide more reactive surface groups for carboxyl-to-amine immobilization of a antibody that is specific against S. aureus. The antibody-enabled EO NCs (Ab@EO NCs) demonstrated 2-fold higher bactericidal efficacy against the targeted bacterium compared to the pristine EO NCs at the same concentrations. The improved antibacterial effect of the Ab@EO NCs toward S. aureus was also confirmed in a real-time assay by monitoring bacterial cells elimination using a quartz crystal microbalance. Furthermore, the Ab@EO NCs selectively decreased the load and changed the cell morphology of the targeted S. aureus in a mixed inoculum with nontargeted Pseudomon...Continue Reading

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Citations

Mar 7, 2021·Polymers·Vera Alexandra SpirescuEcaterina Andronescu

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Methods Mentioned

BETA
quartz crystal microbalance
Florescence microscopy
transmission electron microscopy
scanning electron microscopy
fluorescence microscopy
QCM-D

Software Mentioned

ImageJ
NTA

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